This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Dillman, R. O. Articles by Royston, I. Search for Related Content PubMed PubMed Citation Articles by Dillman, R. O. Articles by Royston, I.

Establishment and Characterization of an Epstein-Barr Virus-negative Lymphoma B-Cell Line from a Patient with a Diffuse Large Cell Lymphoma¹

Division of Hematology/Oncology, Department of Medicine, University of California, San Diego, School of Medicine, and Veterans Administration Medical Center, La Jolla, California 92093

Cell line (LNPL) was established from a tumor biopsy of a nasopharyngeal "histiocytic" lymphoma composed of large noncleaved B-cells. LNPL expressed immunoglobulin M surface immunoglobulin, as did the original tumor. LNPL also expressed la antigen but lacked Epstein-Barr virus nuclear antigen. It exhibited a translocation between chromosomes 8 and 14 which has been described in certain other malignant lymphoproliferative disorders. An autologous T-cell line lacked the translocation between chromosomes 8 and 14. LNPL is being used as an immunogen to produce monoclonal antibodies in our laboratory. Such cell lines may facilitate identification of lymphoma-associated surface antigens.

¹ Supported by the Veterans Administration, Grant IM 207 of the American Cancer Society, Contract N01-CB-84250-31 of the National Cancer Institute, and NIH Grant PHSHL-07107-05.

² To whom requests for reprints should be addressed, at Division of Hematology/Oncology—V111E, University of California, San Diego, School of Medicine, La Jolla, Calif. 92093.

Received 3/17/81. Accepted 1/ 4/82.