This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chang, S. E. Articles by Taylor-Papadimitriou, J. Search for Related Content PubMed PubMed Citation Articles by Chang, S. E. Articles by Taylor-Papadimitriou, J.

Establishment and Characterization of SV40-transformed Human Breast Epithelial Cell Lines

Imperial Cancer Research Fund, P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, United Kingdom

Human breast epithelial cells cultured from milk have been transformed with SV40. Indirect immunofluorescence tests using monoclonal antibodies show that cells from clones grown in soft agar have SV40 large T-antigen in their nuclei and epithelia-specific tonofilament antigens on their intermediate filaments. In primary cultures of milk epithelial cells, the tonofilaments form a characteristic delicate basketwork throughout the cytoplasm, but in the SV40-transformed epithelial cell strains, the filament network is grossly distorted. Insulin, hydrocortisone, and serum stimulate the growth of the cell strains. At passages 8 to 11, the cell strains become quiescent and usually die. One cell strain survived this crisis period and gave rise to the fR series of cell lines. Most cell lines have a cuboidal morphology and react with a monoclonal antibody that recognizes a differentiation antigen on the membranes of breast epithelia. Line fR2 expressed the highest level of this antigen whereas fR5, the only fR line isolated with fusiform morphology, had relatively little. The in vitro-transformed lines may be related to the two dominant epithelial cell types seen in primary milk cultures and could be useful for studying the relationship between transformation and differentiation in human mammary epithelial cells.

Received 6/ 9/81. Accepted 1/27/82.

This article has been cited by other articles:

				Y. T. Kwon, S. A. Balogh, I. V. Davydov, A. S. Kashina, J. K. Yoon, Y. Xie, A. Gaur, L. Hyde, V. H. Denenberg, and A. Varshavsky Altered Activity, Social Behavior, and Spatial Memory in Mice Lacking the NTAN1p Amidase and the Asparagine Branch of the N-End Rule Pathway Mol. Cell. Biol., June 1, 2000; 20(11): 4135 - 4148. [Abstract] [Full Text]

				F Berdichevsky, D Alford, B D'Souza, and J Taylor-Papadimitriou Branching morphogenesis of human mammary epithelial cells in collagen gels J. Cell Sci., January 12, 1994; 107(12): 3557 - 3568. [Abstract] [PDF]

				F Castro-Munozledo Development of a spontaneous permanent cell line of rabbit corneal epithelial cells that undergoes sequential stages of differentiation in cell culture J. Cell Sci., January 8, 1994; 107(8): 2343 - 2351. [Abstract] [PDF]