This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Messing, E. M. Articles by Bubbers, J. E. Search for Related Content PubMed PubMed Citation Articles by Messing, E. M. Articles by Bubbers, J. E.

Serum-free Medium for the in Vitro Growth of Normal and Malignant Urinary Bladder Epithelial Cells¹

Division of Urology, Department of Surgery [E. M. M., J. B. D.], and the Departments of Microbiology and Immunology [J. L. F.], Radiation Oncology [J. E. B.], and Pathology [S. M. B.], UCLA School of Medicine, Los Angeles, California 90024

A serum-free medium, DH-S1, is described which is valuable for the establishment of primary cultures of normal and malignant transitional epithelium (transitional cell carcinoma of the bladder). Growth of epithelial cells in DH-S1 is facilitated but that of fibroblasts is suppressed. Another established human transitional cell carcinoma cell line, 647V, has grown continually in DH-S1 for over 36 passages. Morphological and antigenic studies comparing 647V cells growing in serum-containing and serum-free medium reveal differences which are pronounced at low cell density but which almost disappear at higher densities. A new human transitional cell carcinoma cell line, LA-B1, is described whose initial growth was supported by this serum-free medium.

¹ This work was supported by USPHS Grants 16880, CA 09120, and CA 12880 awarded by the National Cancer Institute, Department of Health and Human Services, and by the Concern Foundation of Los Angeles, Calif. 90024.

² Present address, Division of Urology/Department of Surgery, University of Wisconsin School of Medicine, 600 Highland Avenue, Madison, Wisconsin 53792.

Received 10/26/81. Accepted 3/12/82.

This article has been cited by other articles:

				S. Huguenin, F. Vacherot, L. Kheuang, J. Fleury-Feith, M.-C. Jaurand, M. Bolla, J.-P. Riffaud, and D. K. Chopin Antiproliferative effect of nitrosulindac (NCX 1102), a new nitric oxide-donating non-steroidal anti-inflammatory drug, on human bladder carcinoma cell lines Mol. Cancer Ther., March 1, 2004; 3(3): 291 - 298. [Abstract] [Full Text]