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Chemotaxis of Aortic Endothelial Cells in Response to Fibronectin¹

Departments of Ophthalmology and Physiology, School of Medicine, University of Southern California, and Estelle Doheny Eye Foundation, Los Angeles, California 90033

We have determined the chemotactic response of bovine aortic endothelial cells to fibronectin and to endothelial cell mitogens (endothelial cell growth supplement, tumor extract), using blind-well chemotaxis chambers. Fibronectin induced a chemotactic response in bovine aortic endothelial cells; at 100 µg/ml, chemotaxis increased by 440% above that observed in negative controls (p < 0.001). The chemotactic response plateaued with time, paralleling the disappearance of the fibronectin concentration gradient in the chambers. As further evidence that chemotaxis was measured, we observed that cell migration did not occur when cells were incubated with fibronectin in the absence of a concentration gradient. Endothelial cell mitogens increased bovine aortic endothelial cell proliferation in our experiments but did not stimulate chemotaxis above background levels. In contrast, fibronectin inhibited cell proliferation by 23%. We present a hypothetical model of the role of fibronectin in evoking endothelial cell chemotaxis during tumor neovascularization.

¹ This work was supported by NIH Grants DE-04079 and EY-03229 and by an Institutional Research Award to the Comprehensive Cancer Center of the University of Southern California from the American Cancer Society. The results have been presented, in part, at the Annual Meeting of the American Society for Cell Biology, Cincinnati, Ohio, November 1980 (3).

² To whom requests for reprints should be addressed.

Received 4/28/81. Accepted 3/31/82.

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