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Establishment and Characterization of Two Human Pancreatic Cancer Cell Lines Tumorigenic in Athymic Mice¹

Departments of Medicine [D. L. D., G. M. M., P. A. M., M. D. T., P. C.] and Pathology [G. A. J., H. A. B.] Roger Williams General Hospital, V. A. Medical Center [P. A. M., M. D. T.], and Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912

Two human pancreatic cancer lines, RWP-1 and RWP-2, have been established from 2 patients with primary pancreatic cancer metastatic to the liver. The patients' tumors, the xenografted tumors, and tumors obtained by inoculation of nude mice with cultured RWP-1 and RWP-2 cells are all moderately-well-differentiated ductal cell adenocarcinomas. Ultrastructural analysis supports the tissue histopathology findings. Xenografts of RWP-1 tumors double every 10 days, whereas the doubling time of RWP-2 xenografts is 22 days. Both tumors contain mucin. RWP-1 and RWP-2 cells have a doubling time in culture of 45 hr and form colonies in soft agar. RWP-1 cultures appear to be morphologically heterogeneous; two distinct epithelial cell types can be identified. RWP-1 and RWP-2 have modal chromosome numbers of 64 and 62, respectively. Appreciable levels of glucose-6-phosphate dehydrogenase and lactic dehydrogenase were found in both cell lines and xenografts. RWP-1 and RWP-2 cells produce appreciable amounts of carcinoembryonic antigen, 1090 and 414 ng/10⁶ cells, respectively.

¹ This work was supported by Grants CA 13943, CA20892, and CA30035 and by grants from the Veterans Administration and the Rhode Island Foundation.

² To whom requests for reprints should be addressed, at Department of Medicine, Roger Williams General Hospital, 825 Chalkstone Avenue, Providence, R. I. 02908.

Received 7/23/81. Accepted 4/13/82.

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