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5-Fluorouracil Incorporation in DNA of Human Breast Carcinoma Cells¹

Division of Medicine, Sidney Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115

We have demonstrated previously the presence of 5-fluorouracil (FUra) residues in L1210 DNA. These findings have been extended to the MCF-7 human breast carcinoma cell line. Cesium sulfate gradient centrifugation has been used to separate the MCF-7 RNA and DNA fractions. Alkali and RNase digests have also been used to remove any possible RNA contaminating the DNA fraction. The purified DNA has been analyzed by high-pressure liquid chromatography following digestion to nucleotides and nucleosides. The results demonstrate that FUra residues are detectable in the DNA of these human breast carcinoma cells following exposure to either FUra or 5-fluorodeoxyuridine. Further, the extent of FUra incorporation in both MCF-7 RNA and DNA is similar with either fluorinated pyrimidine. We also demonstrate that the FUra incorporation in DNA from this human cell line can be enhanced by concurrent incubation with thymidine.

¹ This investigation was supported by USPHS Grants CA-28488 and CA-19589 awarded by the National Cancer Institute, Department of Health and Human Services.

² Fellow of the Medical Research Council of Canada.

³ Recipient of an American Cancer Society Junior Faculty Research Award. To whom requests for reprints should be addressed, at the Sidney Farber Cancer Institute, 44 Binney Street, Boston, Mass. 02115.

Received 1/11/82. Accepted 4/27/82.

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