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Methionine Dependency of Cell Growth in Normal and Malignant Hematopoietic Cells¹

Departments of Hematology [Y. K., S. S., K. K., K. H., K. S., F. T.] and Medical Biology [T. K.], and Division of Hemopoiesis, Hematology Research Institute [K. O. Y. M.], Jichi Medical School, Minamikawachi-machi, Tochigi, Japan 329-04

Normal and malignant hematopoietic cell lines, with the exception of MOLT-3, were unable to grow in the methioninedepleted medium which was supplemented with homocysteine. Normal lymphocytes were less stimulated by mitogens in the medium containing homocysteine. Colony-forming units in culture from normal bone marrow cells were not formed in the medium in which homocysteine was substituted for methionine. These data suggest that there are no differences in the methionine requirement for cell growth between normal and malignant hematopoietic cells.

¹ This work was partly supported by a grant-in-aid from the Japanese Ministry of Education.

² To whom requests for reprints should be addressed.

Received 12/ 1/81. Accepted 5/ 7/82.

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