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Unique Cell Surface Glycoprotein Expression in Hairy Cell Leukemia: Effect of Phorbol Ester Tumor Promoters on Surface Glycoproteins, Cell Morphology, and Adherence¹

Departments of Pediatrics and Biochemistry [M. W. L., G. D.] and Medicine [H. M. G.], Joseph P. Kennedy Jr. Mental Retardation Research Center of the Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637

Hairy cell leukemia cells from eight different patients exhibited a characteristic cell surface glycoprotein pattern when labeled by the neuraminidase-galactose oxidase-NaB³H₄ procedure. The diffuse high-molecular-weight glycoprotein band (M_r 230,000 to 300,000) was not seen in other leukemic cell types and may represent a specific hairy cell leukemia antigen. Hairy cell leukemia cells can be maintained as cell suspension cultures, but treatment with a variety of tumor-promoting phorbol esters caused the cells to adhere to plates, assume a fibroblastic elongated shape, and extend long processes. This dramatic morphological change was not associated with any change in surface glycoproteins.

¹ Supported by USPHS Grants CA-19266-04, HD-04583, and HD-06426 and the Robert English Fund, Snell Family Fund, and Bellman Research Fund.

² To whom requests for reprints should be addressed.

Received 2/ 2/82. Accepted 6/ 7/82.

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