This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lockney, M. W. Articles by Dawson, G. Search for Related Content PubMed PubMed Citation Articles by Lockney, M. W. Articles by Dawson, G.

Unique Cell Surface Glycoprotein Expression in Hairy Cell Leukemia: Effect of Phorbol Ester Tumor Promoters on Surface Glycoproteins, Cell Morphology, and Adherence¹

Departments of Pediatrics and Biochemistry [M. W. L., G. D.] and Medicine [H. M. G.], Joseph P. Kennedy Jr. Mental Retardation Research Center of the Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637

Hairy cell leukemia cells from eight different patients exhibited a characteristic cell surface glycoprotein pattern when labeled by the neuraminidase-galactose oxidase-NaB³H₄ procedure. The diffuse high-molecular-weight glycoprotein band (M_r 230,000 to 300,000) was not seen in other leukemic cell types and may represent a specific hairy cell leukemia antigen. Hairy cell leukemia cells can be maintained as cell suspension cultures, but treatment with a variety of tumor-promoting phorbol esters caused the cells to adhere to plates, assume a fibroblastic elongated shape, and extend long processes. This dramatic morphological change was not associated with any change in surface glycoproteins.

¹ Supported by USPHS Grants CA-19266-04, HD-04583, and HD-06426 and the Robert English Fund, Snell Family Fund, and Bellman Research Fund.

² To whom requests for reprints should be addressed.

Received 2/ 2/82. Accepted 6/ 7/82.

This article has been cited by other articles:

				F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leukoc. Biol., November 1, 2005; 78(5): 1025 - 1042. [Abstract] [Full Text] [PDF]

				T. Machii, M. Yamaguchi, R. Inoue, Y. Tokumine, H. Kuratsune, H. Nagai, S. Fukuda, K. Furuyama, O. Yamada, Y. Yahata, et al. Polyclonal B-Cell Lymphocytosis With Features Resembling Hairy Cell Leukemia-Japanese Variant Blood, March 15, 1997; 89(6): 2008 - 2014. [Abstract] [Full Text] [PDF]