This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Graves, D. T. Articles by Antoniades, H. N. Search for Related Content PubMed PubMed Citation Articles by Graves, D. T. Articles by Antoniades, H. N.

Evidence That a Human Osteosarcoma Cell Line Which Secretes a Mitogen Similar to Platelet-derived Growth Factor Requires Growth Factors Present in Platelet-poor Plasma¹

Department of Periodontology, Harvard School of Dental Medicine [D. T. G.], Department of Nutrition, Harvard School of Public Health [A. J. O., H. N. A.], and the Center for Blood Research [H. N. A.], Boston, Massachusetts 02115

A human osteosarcoma-derived cell line, 2T, grows almost as well in medium supplemented with platelet-poor plasma (PPP) as it does in medium containing fetal bovine serum. Human diploid fibroblasts, in contrast, will not grow in medium containing PPP unless human platelet-derived growth factor (PDGF) is added. PPP treated with carboxymethyl-Sephadex at pH 7.4 was able to support 2T cell proliferation, although at a reduced rate compared to untreated PPP. Addition of PDGF to carboxymethyl-Sephadex-treated PPP did not restore the growth rate. However, insulin-like growth factor isolated from human plasma did partially restore the activity of carboxymethyl-Sephadex-treated PPP. Medium conditioned by 2T cells was mitogenic for quiescent BALB/c 3T3 cells and human diploid fibroblasts. Antiserum to human PDGF blocked the mitogenic activity of the conditioned medium. Partial characterization confirmed the biochemical similarity to PDGF. The data are consistent with the hypothesis that these osteosarcoma-derived cells have growth factor requirements similar to those of normal mesenchymal cells but are able to overcome the normal growth limitations by autocrine secretion of PDGF-like mitogens.

¹ Supported by USPHS Grants HL27607 and CA-30101 and the Council for Tobacco Research.

² Recipient of Training Grant 5T32-DE07010.

³ To whom requests for reprints should be addressed.

Received 4/14/82. Accepted 10/12/82.

This article has been cited by other articles:

				D. Graves, A. Owen, R. Barth, P Tempst, A Winoto, L Fors, L. Hood, and H. Antoniades Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells Science, November 23, 1984; 226(4677): 972 - 974. [Abstract] [PDF]

				A. Owen, P Pantazis, and H. Antoniades Simian sarcoma virus--transformed cells secrete a mitogen identical to platelet-derived growth factor Science, July 6, 1984; 225(4657): 54 - 56. [Abstract] [PDF]

				R. Doolittle, M. Hunkapiller, L. Hood, S. Devare, K. Robbins, S. Aaronson, and H. Antoniades Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor Science, July 15, 1983; 221(4607): 275 - 277. [Abstract] [PDF]