This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Anderson, R. Articles by Bagby, G. C. Search for Related Content PubMed PubMed Citation Articles by Anderson, R. Articles by Bagby, G. C., Jr.

Dehydroascorbate Uptake as an in Vitro Biochemical Marker of Granulocyte Differentiation¹

The Hematopoiesis Research Laboratory and Veterans Administration Medical Center, E. E. Osgood Memorial Center, Department of Medicine, Oregon Health Sciences University, Portland, Oregon 97201

We tested the hypothesis that the rate of cellular uptake of dehydroascorbate in cultures of developing granulocyte-macrophage progenitors in vitro would serve as a biochemical marker of neutrophil maturation. Suspension cultures of low-density, nonadherent, T-lymphocyte-depleted bone marrow cells from eight normal volunteers were cultured in medium containing 10% human placental conditioned medium and were harvested at intervals over 14 days. The harvested cells were tested for their ability to take up dehydroascorbate. Mean cellular uptake rate increased 12-fold by Day 10, at which time the cells had differentiated to neutrophils. Uptake increased by less than 2-fold in cells which had been induced to differentiate to mature mononuclear phagocytes with 12-O-tetradecanoylphorbol-13-acetate. Additional studies using HL-60 cells induced to differentiate with dimethyl sulfoxide or 12-O-tetradecanoylphorbol-13-acetate support the view that a major increase in dehydroascorbate uptake in cultured granulopoietic progenitors is a manifestation of a neutrophil differentiation.

¹ Supported in part by the Medical Research Service of the United States Veterans Administration, by NIH Grant AM27483, and by the Medical Research Foundation of Oregon.

² Recipient of Career Development Awards from the Veterans Administration.

³ Recipient of Career Development Award from the Veterans Administration. To whom requests for reprints should be addressed, at Hematopoiesis Research Laboratory, Veterans Administration Medical Center, 3710 S. W. United States Veterans Hospital Road, Portland, Ore. 97201.

Received 4/12/82. Accepted 7/13/83.

This article has been cited by other articles:

				V. H. Guaiquil, C. M. Farber, D. W. Golde, and J. C. Vera Efficient Transport and Accumulation of Vitamin C in HL-60 Cells Depleted of Glutathione J. Biol. Chem., April 11, 1997; 272(15): 9915 - 9921. [Abstract] [Full Text] [PDF]

				J. C. Vera, C. I. Rivas, F. V. Velásquez, R. H. Zhang, I. I. Concha, and D. W. Golde Resolution of the Facilitated Transport of Dehydroascorbic Acid from Its Intracellular Accumulation as Ascorbic Acid J. Biol. Chem., October 6, 1995; 270(40): 23706 - 23712. [Abstract] [Full Text] [PDF]