This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paradies, G. Articles by Papa, S. Search for Related Content PubMed PubMed Citation Articles by Paradies, G. Articles by Papa, S.

Transport of Pyruvate in Mitochondria from Different Tumor Cells¹

Institute of Biological Chemistry, Faculty of Medicine [F. C., S. P.], Centre for the Study of Mitochondria and Energy Metabolism, C.N.R., University of Bari [G. P.], Bari, and Institute of General Pathology, Catholic University, Rome [G. P., T. G.], Italy

A comparative study of the transport of pyruvate in mitochondria isolated from normal rat liver and from three tumors has been carried out. The K_m for net pyruvate uptake in mitochondria isolated from Ehrlich ascites tumor cells is practically equal to that measured in normal rat liver mitochondria while, on the other hand, it is higher in Morris hepatomas 44 and 3924A. The V_max of pyruvate uptake is depressed in all three types of tumor mitochondria as compared to that in the rat liver mitochondria, with the depression being higher in Morris hepatoma 3924A mitochondria. The lower activity of pyruvate translocator in mitochondria isolated from tumor cells as compared to that in rat liver mitochondria is also shown by depression of the rate of pyruvate-supported oxygen uptake. The results document a decreased activity of the pyruvate translocator in tumor mitochondria which seems to be correlated with the growth rate of the tumor cells.

¹ This work has been supported by Grants 78.02854.96, 80.01606.96, and 80.01659.96 from Progetto Finalizzato Controllo della Crescita Neoplastica, Consiglio Nazionale delle Ricerche, Rome, Italy.

² To whom requests for reprints should be addressed.

Received 2/ 3/82. Accepted 7/27/83.

This article has been cited by other articles:

				H. Lu, R. A. Forbes, and A. Verma Hypoxia-inducible Factor 1 Activation by Aerobic Glycolysis Implicates the Warburg Effect in Carcinogenesis J. Biol. Chem., June 21, 2002; 277(26): 23111 - 23115. [Abstract] [Full Text] [PDF]