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Induction of Transformation by Six Classes of Chemical Carcinogens in Adult Rat Liver Epithelial Cells¹

Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, New York 10595

A transformation assay system using two clonal strains of adult rat liver epithelial cells is described. The carcinogens N-methyl-N'-nitro-N-nitrosoguanidine, methylmethanesulfonate, N-2-fluorenylacetamide, aflatoxin B₁, benzo(a)pyrene, dimethylnitrosamine, nitrosopyrrolidine, and dimethyl-4-aminoazobenzene induced anchorage independency in one or both clonal strains, whereas noncarcinogenic analogues were inactive. In the absence of carcinogen exposure, the cell strains exhibited no spontaneous transformation to anchorage independency. These results demonstrate the reliability of anchorage independency as an in vitro end point and the specific responsiveness of adult rat liver epithelial cells to a wide range of carcinogens. Thus, the assay of transformation in adult rat liver epithelial cells is potentially useful for detection of chemical carcinogens and the study of their mechanisms of action.

¹ This study was supported by Contract N01-CP-55705 from the National Cancer Institute and by Contract No. 68-01-6179 from the Environmental Protection Agency.

² To whom requests for reprints should be addressed.

Received 2/ 8/82. Accepted 8/ 1/83.