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Isolation and Partial Purification of a New Class of Transforming Growth Factors from an Avian Sarcoma Virus-transformed Rat Cell Line¹

Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo [R. H., K. Y.], and Faculty of Science, Niigata University, Niigata, Niigata [H. M.], Japan

Transformation of rat cells by avian sarcoma viruses induced the release of growth factors into serum-free conditioned medium. An avian sarcoma virus-transformed rat cell line, 77N1, produced and released a polypeptide growth factor, classified as a transforming growth factor (TGF), which transiently promotes anchorage-dependent BALB3T3 A31 cells to form progressively growing colonies in soft agar. The TGF was isolated and partially purified from an extract of 77N1 cells by ionexchange chromatography on a diethylaminoethyl Sephacel column followed by ammonium sulfate precipitation. The TGF was assumed to have a molecular weight of 11,000 from gel filtration on Sephadex G-50. This TGF did not compete with epidermal growth factor for binding to cell membrane receptors and was not potentiated by epidermal growth factor. The TGF was trypsin and dithiothreitol sensitive as well as heat and acid labile, indicating that it was different from previously reported TGFs of similar molecular weight and thus belonged to a new class of TGFs.

¹ This work was supported by grants from the Ministry of Education, Science, and Culture of Japan.

² To whom requests for reprints should be addressed, at 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113, Japan.

Received 4/28/83. Accepted 8/29/83.

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