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Release of 3-Methyladenine from Linker and Core DNA of Chromatin by a Purified DNA Glycosylase¹

Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

Oligonucleosomes were isolated from [¹⁴C]thymidine-labeled HeLa cells by digestion of the nuclei with micrococcal nuclease and were then alkylated with [³H]methylnitrosourea. Nucleosome core particles were also prepared by further digestion of the oligonucleosomes. The distribution of ³H-labeled methyl groups in the linker versus the core DNA was established by a determination of ³H:¹⁴C ratios in oligonucleosome and core DNA. The ratios in the core DNA of 145 and 165 base pair DNA fragments were 5.2 and 5.4, respectively, while the ratio in the oligonucleosomal DNA was 8.2. Assuming an equal mixture (as determined) of 145 and 165 base pair fragments of DNA in the 185 base pair repeat, the relative concentration of ³H methyl groups in the linker versus the core DNA was 4.2. Thus, 45% of the ³H methyl groups were in the linker DNA, and 55% were in the core DNA. Some shielding of the DNA was evident during alkylation. The concentrations of alkyl groups on the linker and core DNA were 67 and 12% of that found on free DNA alkylated under comparable conditions. No evidence for preferential shielding of the major or minor groove was observed. The purified 3-methyladenine DNA glycosylase I of Escherichia coli released approximately 37% of the 3-methyladenine from the linker DNA and 13% from the core DNA. The limited enzymatic removal of 3-methyladenine in vitro compared to the efficient removal in vivo suggests that conformational changes of the oligonucleosome and core structure must occur for total repair.

¹ Supported by grants from NIH (CA 27528 and GM 22920) and from the Department of Energy (DE-ACO2-76EVO2725).

² NIH Research Career Award Fellowship (5-KO6-GM-21444). To whom requests for reprints should be addressed.

Received 1/17/83. Accepted 8/30/83.