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Effects of Nitrous Oxide on Human Cell Lines¹

Department of Hematology [Y. K., S. S., K. H., K. S., F. T.], Anesthesiology [K. S., T. K.], and Medical Biology [T. K.], National Foundation for Cancer Research [F. T.], Jichi Medical School, Minamikawachi-machi, Tochigi, Japan 329-04

Hematopoietic and nonhematopoietic cell lines cultured in media containing either 5-methyltetrahydrofolate or 5-formyltetrahydrofolate grow well to the same extent. However, when these same cell lines are grown in the presence of nitrous oxide, selective growth inhibition can be shown for hematopoietic cells cultured in 5-methyltetrahydrofolate-containing media. These cells also demonstrated a decreased ability to suppress [³H]-thymidine incorporation into DNA in deoxyuridine suppression tests.

¹ This work was partly supported by a Grant-in-aid from the Japanese Ministry of Education.

² To whom requests for reprints should be addressed.

³ Present address: Third Department of Internal Medicine, Tokyo University, Hongo, Tokyo, Japan.

Received 3/ 1/82. Accepted 12/10/82.