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Monoclonal Antibodies (F36/22 and M7/105) to Human Breast Carcinoma¹

Departments of Diagnostic Immunology Research and Biochemistry [L. D. P., G. A. C., M. J. O., L. A. V., T. M. C.] and Breast Surgery [T. N.], Roswell Park Memorial Institute, Buffalo, New York 14263

Cloned hybridoma cell lines were obtained from fusions of murine myeloma cells with lymphocytes of mice immunized against human breast cancer cells. Hybridomas F36/22 and M7/105 produced antibodies whose binding to breast cancer cells could not be inhibited by prior absorptions with fibroblasts, lymphoblastoid cells, or erythrocytes. Results from cell surface binding assays using a panel of tumor cell lines indicated that antibodies F36/22 and M7/105 recognized determinants expressed maximally on breast cancer cells. Antibody F36/22 reacted with normal mammary epithelial membranes and milk fat globule membranes, whereas antibody M7/105 produced no detectable binding to these specimens. Antigens carrying these epitopes each showed reactivity with concanavalin A lectin. The determinant corresponding to antibody F36/22 was detectable in histological sections of a subset of breast tumors obtained at surgery.

¹ Supported in part by NIH Grants CA-30370 and CA-25653.

² Recipient, National Cancer Institute New Investigator Research Award. To whom requests for reprints should be addressed.

Received 4/ 6/82. Accepted 1/ 7/83.