This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lotan, R. Articles by Raz, A. Search for Related Content PubMed PubMed Citation Articles by Lotan, R. Articles by Raz, A.

Low Colony Formation in Vivo and in Culture as Exhibited by Metastatic Melanoma Cells Selected for Reduced Homotypic Aggregation

Departments of Biophysics [R. L.] and Cell Biology [A. R.], The Weizmann Institute of Science, Rehovot, Israel 76100

A subpopulation of cells unable to aggregate in the presence of a high concentration of asialofetuin (400 µg/ml) has been isolated from the murine B16-F1 melanoma cells which aggregate readily at low asialofetuin concentrations (>0.3 µg/ml). Cells of this variant cell line, designated B16-F1-NA, exhibited also a reduced tendency to undergo homotypic aggregation in the presence of syngeneic serum. In culture, the B16-F1-NA cells spread on solid substrata more than the B16-F1, formed more focal contacts, and proliferated at a slower exponential rate. The pattern of the major cell surface proteins and glycoproteins was similar in the parental and variant cells except for a minor glycoprotein with a molecular weight of 150,000 which was labeled more intensely on the B16-F1 than on the B16-F1-NA cells. Colony formation in semisolid medium and the development of experimental metastases in the lungs of syngeneic mice were markedly reduced in the B16-F1-NA as compared with the parental cells. It is suggested that the ability to undergo aggregation in the presence of glycoproteins is an important property of malignant cells which may influence anchorage-independent growth and the formation of metastases.

¹ Supported by a grant from the United States-Israel Binational Science Foundation. To whom requests for reprints should be addressed.

² Supported by the Israel Cancer Research Fund of New York.

Received 2/24/82. Accepted 1/20/83.

This article has been cited by other articles:

				M. Tan, R. Grijalva, and D. Yu Heregulin {beta}1-activated Phosphatidylinositol 3-Kinase Enhances Aggregation of MCF-7 Breast Cancer Cells Independent of Extracellular Signal-regulated Kinase Cancer Res., April 1, 1999; 59(7): 1620 - 1625. [Abstract] [Full Text] [PDF]

				M. Hejna, M. Raderer, and C. C. Zielinski Inhibition of Metastases by Anticoagulants J Natl Cancer Inst, January 6, 1999; 91(1): 22 - 36. [Abstract] [Full Text] [PDF]

				A Raz and A Ben-Ze'ev Modulation of the metastatic capability in B16 melanoma by cell shape Science, September 23, 1983; 221(4617): 1307 - 1310. [Abstract] [PDF]