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Correlation of Amino Acid Fucoside Labeling Patterns with Tumorigenicity of Mouse Mammary Epithelial Cells¹

Department of Medical Microbiology and Immunology, College of Medicine, University of Kentucky, Lexington, Kentucky 40536 [M. R. S.], and Departments of Virology and Epidemiology [C. S. R., J. S. B.] and Cell Biology [D. M.], Baylor College of Medicine, Houston, Texas 77030

The amino acid fucosides of tumorigenic and nontumorigenic mouse mammary gland-derived cells were studied. The cells examined included tumorigenic cell lines derived from mammary carcinomas of the following etiologies: induction by hormone; virus; and chemical carcinogen. Also studied were cells derived from normal mammary glands and several clones of cells, which were derived from a mammary carcinoma but were not demonstrably tumorigenic at lower passage levels after cloning, while they were highly tumorigenic at higher passage levels. Cells were cultured in medium supplemented with radiolabeled fucose and extracted, and extracts were analyzed for the amino acid fucosides. Radiolabeled compounds which comigrated with the amino acid fucosides glucosylfucosylthreonine, fucosylthreonine, and fucosylserine were observed. There was a distinctive difference between the tumorigenic and nontumorigenic cells; the ratio of fucosylthreonine plus fucosylserine to glucosylfucosylthreonine was higher in all tumorigenic cells as compared to the ratio observed for the nontumorigenic cells.

¹ Supported by Grant CA25730, Contract 1CB53904, and Biomedical Research Support Grant RR05374, NIH.

² To whom requests for reprints should be addressed.

³ Present address: the Veterans Medical Center and the University Health Science Center at Dallas, Dallas, Texas 75216.

Received 10/ 5/82. Accepted 3/10/83.