This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Chung, L. W. K. Articles by Breitweiser, K. Search for Related Content PubMed PubMed Citation Articles by Chung, L. W. K. Articles by Breitweiser, K.

Comparison of Soluble Protein Kinases from Normal Rat Prostates and Prostatic Dunning Tumors¹

Pharmacology/School of Pharmacy, University of Colorado, Boulder, Colorado 80309 [L. W. K. C., K. B.], and Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262 [L. W. K. C.]

The photoaffinity labeling of the regulatory subunits of adenosine cyclic 3':5'-monophosphate-dependent soluble protein kinases with an adenosine cyclic 3':5' monophosphate analogue, 8-azidoadenosine cyclic 3':5'-monophosphate (8-N₃-cAMP) was compared in normal prostates and prostatic (Dunning) tumors. Marked differences in the proportions of photoincorporation of 8-N₃-[³²P]cAMP into the regulatory subunit of type I (R_I) and type II (R_II) protein kinases and the proteolytic fragment of these regulatory subunits (R_pf) were observed in normal prostate (ventral prostate versus dorsolateral prostate) and Dunning prostatic tumors [hormone-dependent (R-3327-H) versus hormone-independent (R-3327-HI) or anaplastic (R-3327-AT) tumors]. The proportion of 8-N₃-[³²P]cAMP photoincorporation into R_II versus R_I was 2-fold higher in the dorsolateral prostate than that observed in the ventral prostate, whereas the ratio of photoincorporation into R_I or R_pf was comparable between these two tissues. Comparison between tumor tissues revealed that the proportion of photoincorporation of 8-N₃-[³²P]cAMP into R_II versus R_I was 4.2-fold higher in hormone-dependent than in hormone-independent or anaplastic tumors, whereas the ratio of photoincorporation into R_I versus R_pf in the former was only 6 to 7% that found in the latter. Since Dunning hormone-dependent and hormone-independent tumors have similar morphology, the present photoaffinity labeling of these regulatory subunits of soluble protein kinases provides a sensitive method (4 mg tissue per assay) to distinguish these prostatic tumors of different hormonal dependency.

¹ Preliminary results have been presented (9).

² Recipient of USPHS Grant CA-27418.

Received 10/14/82. Accepted 3/29/83.