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Comparison of the Sister Chromatid Exchange and Cell Survival Assays as a Measure of Tumor Cell Sensitivity in Vitro to cis-Diamminedichloroplatinum (II)¹

Brain Tumor Research Center of the Department of Neurological Surgery [P. J. T., M. E. W., M. H. B., D. F. D.], and the Department of Radiation Oncology [D. F. D.], School of Medicine, University of California, San Francisco, California 94143

Cytotoxic effects of treating RIF-1, EMT6, and 9L tumor cell lines with cis-diamminedichloroplatinum(II) were measured with the sister chromatid exchange assay and compared to results obtained with the colony-forming efficiency assay. The greatest number of sister chromatid exchanges was induced in RIF-1 cells, fewer in EMT6 cells, and the least in 9L cells. Cell survival data obtained with the colony-forming efficiency assay paralleled data obtained with the sister chromated exchange assay. These studies suggest that the sister chromatid exchange assay may be a useful method with which to determine the in vitro sensitivity of tumor cells to some antineoplastic agents.

¹ This research was supported by NIH Grants CA-31867, CA-09215, and Program Project Grant CA-13525, and by the Aaron Silvera Fund.

Received 10/ 8/82. Accepted 4/26/83.