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Department of Medicine, University of Texas Health Science Center, San Antonio, Texas 78284 [C. K. O., D. H. B., G. M. C.], and the University of Arizona Health Science Center, Tuscon, Arizona 85724 [J. M. T.]
We have studied the effects of tamoxifen on the cell cycle kinetics of the endocrine-responsive MCF-7 human breast cancer cells. Tamoxifen inhibits proliferation of MCF-7 cells. The tritiated thymidine labeling index is markedly reduced by tamoxifen, indicating a reduction in the fraction of cells in S phase. Flow cytometry of mithramycin-stained cells reveals that cells accumulate in G1 phase, with a concomitant depletion of S- and G2-M-phase cells with tamoxifen. Mapping of G1-phase cells by morphology of prematurely condensed chromosomes demonstrated that tamoxifen-treated cells accumulate in early G1. These studies indicate that tamoxifen inhibits proliferation of MCF-7 human breast cancer cells by invoking a transition delay early in the G1 phase of the cell cycle.
1 This work was supported by NIH Grant CA 30251 and by an institutional grant from the American Cancer Society (IN-116C).
2 To whom requests for reprints should be addressed.
3 Recipient of National Cancer Institute Grants CA 29476 and CA 23074.
Received 12/ 9/82. Accepted 5/ 4/83.
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