This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dipple, A. Articles by Costantino, N. Search for Related Content PubMed PubMed Citation Articles by Dipple, A. Articles by Costantino, N.

Evidence That Binding of 7,12-Dimethylbenz(a)anthracene to DNA in Mouse Embryo Cell Cultures Results in Extensive Substitution of both Adenine and Guanine Residues¹

Basic Research Program-LBI, Chemical Carcinogenesis Program, Frederick Cancer Research Facility, Frederick, Maryland 21701

Primary mouse embryo cell cultures were grown in the presence of [¹⁴C]guanine, labeling primarily deoxyguanosine residues in the cellular DNA, or in the presence of [¹⁴C]adenine, labeling both deoxyguanosine and deoxyadenosine residues in the cellular DNA. These cultures were subsequently exposed to 7,12-[³H]dimethylbenz(a)anthracene for 24 hr. The DNA was isolated and hydrolyzed to deoxyribonucleosides, and the 7,12-dimethylbenz(a)anthracene:deoxyribonucleoside adducts were separated chromatographically allowing the three major adducts found to be identified as bay-region anti-dihydrodiol-epoxide:deoxyguanosine and :deoxyadenosine adducts and a bayregion syn-dihydrodiol-epoxide:deoxyadenosine adduct. Therefore, in contrast to what is known for benzo(a)pyrene, substantial amounts of deoxyadenosine adducts are formed with the more potent carcinogen, 7,12-dimethylbenz(a)anthracene.

¹ Research sponsored by the National Cancer Institute, Department of Health and Human Services, under Contract N01-CO-23909 with Litton Bionetics, Inc. The contents of this publication do not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the United States Government.

² To whom requests for reprints should be addressed.

Received 1/20/83. Accepted 5/31/83.

This article has been cited by other articles:

				M. V. Nesterova and Y. S. Cho-Chung Antisense Protein Kinase A RI{alpha} Inhibits 7,12-Dimethylbenz(a)anthracene-Induction of Mammary Cancer: Blockade at the Initial Phase of Carcinogenesis Clin. Cancer Res., July 1, 2004; 10(13): 4568 - 4577. [Abstract] [Full Text] [PDF]

				T. L. Weimer, A. P. Reddy, U. Harttig, D. Alexander, S. C. Stamm, M. R. Miller, W. Baird, J. Hendricks, and G. Bailey Influence of {beta}-Naphthoflavone on 7,12-Dimethylbenz(a)anthracene Metabolism, DNA Adduction, and Tumorigenicity in Rainbow Trout Toxicol. Sci., October 1, 2000; 57(2): 217 - 228. [Abstract] [Full Text] [PDF]

				M.-s. Tang, S. V. Vulimiri, A. Viaje, J. X. Chen, D. S. Bilolikar, R. J. Morris, R. G. Harvey, T. J. Slaga, and J. DiGiovanni Both ({+/-})syn- and ({+/-})anti-7,12-Dimethylbenz[a]anthracene-3,4-diol-1,2-epoxides Initiate Tumors in Mouse Skin That Possess -CAA- to -CTA- Mutations at Codon 61 of c-H-ras Cancer Res., October 1, 2000; 60(20): 5688 - 5695. [Abstract] [Full Text]

				A. Balmain and C. C.Harris Carcinogenesis in mouse and human cells: parallels and paradoxes Carcinogenesis, March 1, 2000; 21(3): 371 - 377. [Abstract] [Full Text] [PDF]

				V. J. Melendez-Colon, A. Luch, A. Seidel, and W. M. Baird Cancer initiation by polycyclic aromatic hydrocarbons results from formation of stable DNA adducts rather than apurinic sites Carcinogenesis, October 1, 1999; 20(10): 1885 - 1891. [Abstract] [Full Text] [PDF]

				J. M. Arif, W. A. Smith, and R. C. Gupta DNA adduct formation and persistence in rat tissues following exposure to the mammary carcinogen dibenzo[a,l]pyrene Carcinogenesis, June 1, 1999; 20(6): 1147 - 1150. [Abstract] [Full Text] [PDF]

				C. Ip Lessons from Basic Research in Selenium and Cancer Prevention J. Nutr., November 1, 1998; 128(11): 1845 - 1854. [Abstract] [Full Text]

				A. G. McNees, M. O'Donnell, P. H. Horton, H. Y. Kim, S. J. Kim, C. M. Harris, T. M. Harris, and R. S. Lloyd Lack of Correlation between in Vitro and in Vivo Replication of Precisely Defined Benz[a]anthracene Adducted DNAs J. Biol. Chem., December 26, 1997; 272(52): 33211 - 33219. [Abstract] [Full Text] [PDF]

				V. J. Melendez-Colon, C. A. Smith, A. Seidel, A. Luch, K. L. Platt, and W. M. Baird Formation of stable adducts and absence of depurinating DNA adducts in cells and DNA treated with the potent carcinogen dibenzo[a,l]pyrene or its diol epoxides PNAS, December 9, 1997; 94(25): 13542 - 13547. [Abstract] [Full Text] [PDF]