This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Partridge, N. C. Articles by Martin, T. J. Search for Related Content PubMed PubMed Citation Articles by Partridge, N. C. Articles by Martin, T. J.

Morphological and Biochemical Characterization of Four Clonal Osteogenic Sarcoma Cell Lines of Rat Origin¹

Department of Medicine, University of Melbourne, Repatriation General Hospital, Heidelberg, 3081 [N. C. P., V. P. M., T. J. M.], and Department of Anatomy, University of Melbourne, Parkville, 3052 [D. A., G. R.], Victoria Australia

The ultrastructural and biochemical properties of four clonal osteogenic sarcoma lines, UMR 104, 105, 106, and 108, have been compared with uncloned osteogenic sarcoma cells and normal osteoblast-rich cells derived from newborn rat calvaria. High alkaline phosphatase activity and activation of adenylate cyclase by parathyroid hormone were used as biochemical markers of osteoblastic cells. Cloning enriched both of these parameters above those of the parent tumor and far higher than that seen in normal cells, suggesting enrichment of the osteoblast phenotype. Both of these properties have been retained through many passages in culture. Morphologically, the clonal lines have also retained the "blast"-like appearance of the uncloned osteogenic sarcoma cells and consist mainly of flat, relatively featureless cells. Many cells with mitotic figures were observed, indicating continuous cell division taking place in the malignant cells. Each clonal line gave rise to characteristic tumors when reinjected into rats. It is concluded that the clonal osteogenic sarcoma lines are highly differentiated tumor lines which have conserved the differentiated properties of the mature osteoblast, making them a suitable model for the study of the effects of hormones on the growth of a differentiated tumor, as well as for the study of hormonal regulation of the osteoblast.

¹ Supported by grants from the Australian Government Department of Veterans' Affairs, the National Health and Medical Research Council of Australia, and the Children's Leukaemia and Cancer Foundation. The clonal lines can be obtained by qualified investigators from the American Type Culture Collection.

² To whom requests for reprints should be addressed.

Received 12/30/82. Accepted 6/ 7/83.

This article has been cited by other articles:

				A. M. Sorkin, K. C. Dee, and M. L. Knothe Tate "Culture shock" from the bone cell's perspective: emulating physiological conditions for mechanobiological investigations Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1527 - C1536. [Abstract] [Full Text] [PDF]

				M. A. Vargas, M. St-Louis, L. Desgroseillers, J.-L. Charli, and G. Boileau Parathyroid Hormone-Related Protein(1-34) Regulates Phex Expression in Osteoblasts through the Protein Kinase A Pathway Endocrinology, November 1, 2003; 144(11): 4876 - 4885. [Abstract] [Full Text] [PDF]

				H. Samoto, E. Shimizu, Y. Matsuda-Honjyo, R. Saito, S. Nakao, M. Yamazaki, S. Furuyama, H. Sugiya, J. Sodek, and Y. Ogata Prostaglandin E2 Stimulates Bone Sialoprotein (BSP) Expression through cAMP and Fibroblast Growth Factor 2 Response Elements in the Proximal Promoter of the Rat BSP Gene J. Biol. Chem., August 1, 2003; 278(31): 28659 - 28667. [Abstract] [Full Text] [PDF]

				J. L. Fisher, P. S. Mackie, M. L. Howard, H. Zhou, and P. F. M. Choong The Expression of the Urokinase Plasminogen Activator System in Metastatic Murine Osteosarcoma: An in Vivo Mouse Model Clin. Cancer Res., June 1, 2001; 7(6): 1654 - 1660. [Abstract] [Full Text] [PDF]

				R. R. Miles, J. P. Sluka, D. L. Halladay, R. F. Santerre, L. V. Hale, L. Bloem, K. Thirunavukkarasu, R. J. S. Galvin, J. M. Hock, and J. E. Onyia ADAMTS-1: A Cellular Disintegrin and Metalloprotease with Thrombospondin Motifs Is a Target for Parathyroid Hormone in Bone Endocrinology, December 1, 2000; 141(12): 4533 - 4542. [Abstract] [Full Text] [PDF]

				A. Yamaguchi, T. Komori, and T. Suda Regulation of Osteoblast Differentiation Mediated by Bone Morphogenetic Proteins, Hedgehogs, and Cbfa1 Endocr. Rev., August 1, 2000; 21(4): 393 - 411. [Abstract] [Full Text] [PDF]

				A. Wang, J. A. Martin, L. A. Lembke, and R. J. Midura Reversible Suppression of in Vitro Biomineralization by Activation of Protein Kinase A J. Biol. Chem., April 6, 2000; 275(15): 11082 - 11091. [Abstract] [Full Text] [PDF]

				A. Santhanagopal and S. J. Dixon Insulin-like growth factor I rapidly enhances acid efflux from osteoblastic cells Am J Physiol Endocrinol Metab, September 1, 1999; 277(3): E423 - E432. [Abstract] [Full Text] [PDF]

				F. Lecanda, D. A. Towler, K. Ziambaras, S.-L. Cheng, M. Koval, T. H. Steinberg, and R. Civitelli Gap Junctional Communication Modulates Gene Expression in Osteoblastic Cells Mol. Biol. Cell, August 1, 1998; 9(8): 2249 - 2258. [Abstract] [Full Text]

				V. K. Tam, S. Schotland, and J. Green Inflammatory cytokines (IL-1alpha , TNF-alpha ) and LPS modulate the Ca2+ signaling pathway in osteoblasts Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1686 - C1698. [Abstract] [Full Text] [PDF]

				N. Ishikawa, H. Taniguchi-Seto, Y. Munakata, Y. Takagi, N. Shimada, and N. Kimura Multiple Transcripts for Rat Nucleoside Diphosphate Kinase alpha Isoform Are Structurally Categorized into Two Groups That Exhibit Cell-specific Expression and Distinct Translation Potential J. Biol. Chem., February 7, 1997; 272(6): 3289 - 3295. [Abstract] [Full Text] [PDF]

				A. M. Hocking, R. A. Strugnell, P. Ramamurthy, and D. J. McQuillan Eukaryotic Expression of Recombinant Biglycan. POST-TRANSLATIONAL PROCESSING AND THE IMPORTANCE OF SECONDARY STRUCTURE FOR BIOLOGICAL ACTIVITY J. Biol. Chem., August 9, 1996; 271(32): 19571 - 19577. [Abstract] [Full Text] [PDF]

				E. M. Greenfield, M. C. Horowitz, and S. A. Lavish Stimulation by Parathyroid Hormone of Interleukin-6 and Leukemia Inhibitory Factor Expression in Osteoblasts Is an Immediate-early Gene Response Induced by cAMP Signal Transduction J. Biol. Chem., May 3, 1996; 271(18): 10984 - 10989. [Abstract] [Full Text] [PDF]

				E. Mackie and S Ramsey Modulation of osteoblast behaviour by tenascin J. Cell Sci., January 6, 1996; 109(6): 1597 - 1604. [Abstract] [PDF]

				A. Azarani, J. Orlowski, and D. Goltzman Parathyroid Hormone and Parathyroid Hormone-related Peptide Activate the Na[IMAGE]/H[IMAGE] Exchanger NHE-1 Isoform in Osteoblastic Cells (UMR-106) via a cAMP-dependent Pathway J. Biol. Chem., September 29, 1995; 270(39): 23166 - 23172. [Abstract] [Full Text] [PDF]

				C. M. Stanford, P. A. Jacobson, E. D. Eanes, L. A. Lembke, and R. J. Midura Rapidly Forming Apatitic Mineral in an Osteoblastic Cell Line (UMR 106-01 BSP) J. Biol. Chem., April 21, 1995; 270(16): 9420 - 9428. [Abstract] [Full Text] [PDF]

				E. J. Mackie and R. P. Tucker Tenascin in bone morphogenesis: expression by osteoblasts and cell type-specific expression of splice variants J. Cell Sci., November 1, 1992; 103(3): 765 - 771. [Abstract] [PDF]

				J. B. Lian and G. S. Stein Concepts of Osteoblast Growth and Differentiation: Basis for Modulation of Bone Cell Development and Tissue Formation Critical Reviews in Oral Biology & Medicine, January 1, 1992; 3(3): 269 - 305. [Abstract] [Full Text] [PDF]

				B. Kemp, J. Moseley, C. Rodda, P. Ebeling, R. Wettenhall, D Stapleton, H Diefenbach-Jagger, F Ure, V. Michelangeli, H. Simmons, et al. Parathyroid hormone-related protein of malignancy: active synthetic fragments Science, December 11, 1987; 238(4833): 1568 - 1570. [Abstract] [PDF]