This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ashendel, C. L. Articles by Boutwell, R. K. Search for Related Content PubMed PubMed Citation Articles by Ashendel, C. L. Articles by Boutwell, R. K.

Protein Kinase Activity Associated with a Phorbol Ester Receptor Purified from Mouse Brain¹

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706

Protein kinase activity copurified with the receptor for 12-O-[³H]tetradecanoylphorbol-13-acetate during its purification from mouse brain particulate protein. All attempts to resolve the protein kinase activity from the receptor were unsuccessful. The isolated receptor required phospholipid and divalent calcium for maximal portein kinase activity. The protein kinase was not activated by cyclic nucleotides or calmodulin. There are striking similarities between the receptor-associated protein kinase activity and the calcium- and phospholipid-dependent protein kinase, which has been suspected of mediating the effects of biological stimuli associated with increased phosphatidylinositol turnover.

¹ Supported by NIH Grants CA-09135, CA-22484, and CA-07175. This is Paper 2 of 2 articles on the biochemical nature of the phorbol ester receptor. Paper 1 (2) describes the isolation of this receptor from mouse brain particulate protein.

² Present address: Department of Medicinal Chemistry, Pharmacy Building, Purdue University, W. Lafayette, Ind. 47907. To whom requests for reprints should be addressed.

Received 10/28/82. Accepted 6/ 8/83.

This article has been cited by other articles:

				E. Deutsch, A. Cohen, G. Kazimirsky, S. Dovrat, H. Rubinfeld, C. Brodie, and R. Sarid Role of Protein Kinase C {delta} in Reactivation of Kaposi's Sarcoma-Associated Herpesvirus J. Virol., September 15, 2004; 78(18): 10187 - 10192. [Abstract] [Full Text] [PDF]

				M. K. Graham, I. Fairweather, and J. G. McGeown Second messengers mediating mechanical responses to the FARP GYIRFamide in the fluke Fasciola hepatica Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2000; 279(6): R2089 - R2094. [Abstract] [Full Text] [PDF]

				D. RON and M. G. KAZANIETZ New insights into the regulation of protein kinase C and novel phorbol ester receptors FASEB J, October 1, 1999; 13(13): 1658 - 1676. [Abstract] [Full Text]

				H. Wang and R. Smart Overexpression of protein kinase C-alpha in the epidermis of transgenic mice results in striking alterations in phorbol ester-induced inflammation and COX-2, MIP-2 and TNF-alpha expression but not tumor promotion J. Cell Sci., January 10, 1999; 112(20): 3497 - 3506. [Abstract] [PDF]

				M. J. Caloca, N. Fernandez, N. E. Lewin, D. Ching, R. Modali, P. M. Blumberg, and M. G. Kazanietz beta 2-Chimaerin Is a High Affinity Receptor for the Phorbol Ester Tumor Promoters J. Biol. Chem., October 17, 1997; 272(42): 26488 - 26496. [Abstract] [Full Text] [PDF]

				M. G. Kazanietz, N. E. Lewin, J. D. Bruns, and P. M. Blumberg Characterization of the Cysteine-rich Region of the Caenorhabditiselegans Protein Unc-13 as a High Affinity Phorbol Ester Receptor J. Biol. Chem., May 5, 1995; 270(18): 10777 - 10783. [Abstract] [Full Text] [PDF]

				K. Enomoto, A. Kaneko, M. Oyamada, Y. Oyamada, K. Dempo, and M. Mori Induction of a Novel Ca2+-Dependent Serine Protease in Rat Liver Treated with Various Promoters of Liver Carcinogenesis Toxicol Pathol, January 1, 1987; 15(1): 73 - 77. [Abstract] [PDF]

				P. Parker, L Coussens, N Totty, L Rhee, S Young, E Chen, S Stabel, M. Waterfield, and A Ullrich The complete primary structure of protein kinase C--the major phorbol ester receptor Science, August 22, 1986; 233(4766): 853 - 859. [Abstract] [PDF]

				A. Ullrich, H. Riedel, Y. Yarden, L. Coussens, A. Gray, T. Dull, J. Schlessinger, M.D. Waterfield, and P.J. Parker Protein Kinases in Cellular Signal Transduction: Tyrosine Kinase Growth Factor Receptors and Protein Kinase C Cold Spring Harb Symp Quant Biol, January 1, 1986; 51(0): 713 - 724. [Abstract] [PDF]

				P. Cerutti Prooxidant states and tumor promotion Science, January 25, 1985; 227(4685): 375 - 381. [Abstract] [PDF]

				L. McPhail, C. Clayton, and R Snyderman A potential second messenger role for unsaturated fatty acids: activation of Ca2+-dependent protein kinase Science, May 11, 1984; 224(4649): 622 - 625. [Abstract] [PDF]