This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, Y.-c. Articles by Rao, P. N. Search for Related Content PubMed PubMed Citation Articles by Wang, Y.-c. Articles by Rao, P. N.

Effect of Gossypol on DNA Synthesis and Cell Cycle Progression of Mammalian Cells in Vitro¹

Department of Chemotherapy Research, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030

Gossypol, a yellow phenolic compound extracted from cotton plants that is being used as a male antifertility drug, has been found to have cytotoxic effects. The objective of the present study was to determine the cause of these cytotoxic effects in cultured cells and to ascertain whether cells in certain phases of the cell cycle were more sensitive to the drug than were cells in other phases. HeLa or Chinese hamster ovary cells were exposed to various concentrations of gossypol for varying periods of time, and the effects of the drug on the growth rate, cell cycle traverse, plating efficiency, and macromolecular synthesis were measured. The results of this study indicate that gossypol is a specific inhibitor of DNA synthesis; it has no effect on RNA and protein synthesis at a concentration of 10 µg/ml and hence has no effect on the cell cycle traverse of cells from G₂ to the beginning of S phase. In the presence of the drug, cells can enter S phase but fail to complete replication. The effects of the drug on growth rate and plating efficiency indicate that there is a threshold concentration at which gossypol becomes effective as a cytotoxic agent. Gossypol did not increase chromosome aberrations in the treated cells. Since gossypol irreversibly blocks cells in S phase, it could be useful as an antitumor drug.

¹ Supported in part by USPHS Grants CA-27544 and CA-34783, awarded by the National Cancer Institute, Department of Health and Human Services and by Grant CH-205 from the American Cancer Society.

² Present address: Department of Biology, Beijing Normal University, Beijing, People's Republic of China.

³ To whom requests for reprints should be addressed.

Received 7/27/83. Accepted 9/14/83.

This article has been cited by other articles:

				K. Balakrishnan, W. G. Wierda, M. J. Keating, and V. Gandhi Gossypol, a BH3 mimetic, induces apoptosis in chronic lymphocytic leukemia cells Blood, September 1, 2008; 112(5): 1971 - 1980. [Abstract] [Full Text] [PDF]