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Secretion of Erythropoietin-like Activity by Clones of Human Renal Carcinoma Cell Line GKA¹

Division of Hematology and Oncology, Children's Hospital and the Dana-Farber Cancer Institute; Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115 [A. J. S., K. A. B., G. M. S.]; and the Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720 [J. F. G.]

Human renal carcinoma cell line GKA was derived from a patient with the paraneoplastic syndrome of erythrocytosis and secretes erythropoietin-like activity into its growth medium (Sytkowski, A. J., Richie, J. P., and Bicknell, K. A. Cancer Res., 43: 1415–1419, 1983). In order to derive homogeneous sublines with higher secretory rates, we cloned line GKA. Over 100 clones were generated, and 21 secreted erythropoietin-like activity, up to 6-fold higher than the uncloned line. This activity stimulated the growth and differentiation of CFU-E derived colonies in plasma clot culture. However, the secreted erythropoietin-like activity did not cross-react in a sensitive radioimmunoassay utilizing highly purified ¹²⁵I-labeled human urinary erythropoietin and heterologous anti-human urinary erythropoietin antiserum. These results suggest that line GKA secretes an erythropoietic stimulating factor distinct from the hormone erythropoietin.

¹ Supported in part by Grants CA 26105 and AM 29992.

² Recipient of Research Career Development Award AM 01001. To whom requests for reprints should be addressed, at Children's Hospital, 300 Longwood Avenue, Boston, Mass. 02115.

Received 5/10/83. Accepted 9/19/83.