This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Medina, D. Articles by Oborn, C. J. Search for Related Content PubMed PubMed Citation Articles by Medina, D. Articles by Oborn, C. J.

Selenium Inhibition of DNA Synthesis in Mouse Mammary Epithelial Cell Line YN-4¹

Department of Cell Biology, Baylor College of Medicine, Texas Medical Center, Houston, Texas 77030

Previous results have documented that Na₂SeO₃ has a biphasic effect on the growth of mammary cells in vitro. In the experiments reported herein, the effects of selenium on several parameters of cell proliferation in the YN-4 mouse mammary cell line were investigated. The biphasic effect of selenium on cell growth was confirmed; i.e., 5 x 10^-8 M selenium stimulated cell growth, whereas 5 x 10^-6 M delayed cell growth and 5 x 10^-5 M was cytotoxic. The inhibition of cell growth by 5 x 10^-6 M selenium was reversible when this dose was removed from the growth medium. The increased cell growth at 5 x 10^-8 M selenium was reflected by an increased cell number, increased uptake of [³H]thymidine into DNA, increased DNA labeling index, and an increased rate of DNA synthesis. The decreased cell growth at 5 x 10^-6 M selenium was reflected by a decrease in all of these parameters of cell growth kinetics. The differential effects of selenium were manifested by 48 hr after addition of selenium to the cell culture medium. The results indicate that one of the mechanisms of selenium-mediated inhibition of carcinogenesis may be due to an inhibition of cell proliferation of responsive cells.

¹ This study was supported by Research Grant CA 11944 from the National Cancer Institute.

² To whom requests for reprints should be addressed.

Received 11/14/83. Accepted 7/ 2/84.

This article has been cited by other articles:

				N. V. Gopee, V. J. Johnson, and R. P. Sharma Sodium Selenite-Induced Apoptosis in Murine B-Lymphoma Cells Is Associated with Inhibition of Protein Kinase C-{delta}, Nuclear Factor {kappa}B, and Inhibitor of Apoptosis Protein Toxicol. Sci., April 1, 2004; 78(2): 204 - 214. [Abstract] [Full Text] [PDF]

				S. Cao, F. A. Durrani, and Y. M. Rustum Selective Modulation of the Therapeutic Efficacy of Anticancer Drugs by Selenium Containing Compounds against Human Tumor Xenografts Clin. Cancer Res., April 1, 2004; 10(7): 2561 - 2569. [Abstract] [Full Text] [PDF]

				R. Gopalakrishna and U. Gundimeda Antioxidant Regulation of Protein Kinase C in Cancer Prevention J. Nutr., December 1, 2002; 132(12): 3819S - 3823. [Abstract] [Full Text] [PDF]

				C. V. Rao, C-Q. Wang, B. Simi, J. G. Rodriguez, I. Cooma, K. El-Bayoumy, and B. S. Reddy Chemoprevention of Colon Cancer by a Glutathione Conjugate of 1,4-Phenylenebis(methylene)selenocyanate, a Novel Organoselenium Compound with Low Toxicity Cancer Res., May 1, 2001; 61(9): 3647 - 3652. [Abstract] [Full Text]

				H.-S. Park, E. Park, M.-S. Kim, K. Ahn, I. Y. Kim, and E.-J. Choi Selenite Inhibits the c-Jun N-terminal Kinase/Stress-activated Protein Kinase (JNK/SAPK) through a Thiol Redox Mechanism J. Biol. Chem., January 28, 2000; 275(4): 2527 - 2531. [Abstract] [Full Text] [PDF]

				J. Wang, M. Kobayashi, K. Sakurada, M. Imamura, T. Moriuchi, and M. Hosokawa Possible Roles of an Adult T-Cell Leukemia (ATL)-Derived Factor/Thioredoxin in the Drug Resistance of ATL to Adriamycin Blood, April 1, 1997; 89(7): 2480 - 2487. [Abstract] [Full Text] [PDF]