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Prostaglandins in Cells of the Lymphoid System in AKR Leukemia¹

Sloan-Kettering Institute for Cancer Research, New York, New York 10021 [R. A. K., H. T. T.]; Ludwig-Maximilians-Universitat München Klinikum Grosshadern, Marchionininstrasse 15, 8000 München 70, Germany [T. W.]; and the Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma [R. A. G.]

AKR mice develop spontaneous lymphoid leukemia late (8 to 12 months) in life, although persistent murine leukemia virus production occurs throughout their life. This suggests that agerelated changes are involved in development of leukemia. Prostaglandin biosynthesis was therefore studied in 24-hr cultures in vitro at 37° of peritoneal macrophages, splenocytes, thymocytes, bone marrow, and lymph node cells. AKR mice of 2, 6, and 8 to 12 months of age were studied. Prostaglandin E₁, prostaglandin E₂, prostaglandin F₂, 6-ketoprostaglandin F₁, and thromboxane B₂ were measured. In cultures of peritoneal macrophages and cells from spleen, thymus, and lymph nodes, the biosynthesis of all five prostaglandin moieties was higher in those cultures prepared from 8- to 12-month-old spontaneously leukemic mice in comparison with those from 2-month-old nonleukemic AKR mice. However, when leukemia was transplanted in 3-month-old AKR mice, synthesis of all five compounds was reduced significantly in cultures of peritoneal macrophages and splenocytes prepared from these 3-month-old leukemic mice. The present data demonstrate abnormalities in prostaglandin synthesis by various cells of the immune system in leukemic mice. However, the nature of these changes was different in cultures of cells from spontaneously leukemic mice from those with transplanted leukemia. Age-related increases in prostaglandin synthesis by various lymphoid cells from spontaneously leukemic AKR mice (8 to 12 months old) occurred at a much earlier age than in BALB/c mice and may be related to the leukemic condition.

¹ Aided by a grant from the Sherman Fairchild Foundation and the Special Projects Grant from the Sloan-Kettering Institute.

² To whom requests for reprints should be addressed.

Received 4/ 4/83. Accepted 10/24/83.