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Rate of Growth and Extent of Differentiation Reflected by Cytoplasmic Proteins and Antigens of Human Colon Tumor Cell Lines¹

Department of Pharmacology and Bristol-Baylor Laboratory, Baylor College of Medicine, Houston, Texas 77030

The cytosolic proteins and antigens of 11 human colon tumor cell lines were examined with respect to their rate of growth and state of differentiation. Coomassie blue-stained protein analysis of sodium dodecyl sulfate-containing polyacrylamide gels revealed protein bands at M_r 30,000, 31,000, and 58,000, which were characteristic of slower growing and more differentiated cell lines. More rapidly dividing and disdifferentiated colon cell lines lacked the M_r 30,000 and M_r 58,000 bands; instead, they produced a single protein band that ran between the M_r 30,000 and M_r 31,000 positions on the gel.

Western transfer analysis of cytoplasmic antigens further subdivided the 11 cell lines into 3 separate categories. Slowly growing and more differentiated lines produced a M_r 52,000 antigen. Intermediate lines, with respect to growth rate and state of differentiation, produced a M_r 38,000 antigen. The rapidly growing and highly disdifferentiated cell lines contained three cytosolic antigens with molecular weights of 37,000, 39,000, and 48,000.

These criteria made it possible to classify these 11 human colon tumor tissue culture cell lines into 3 groups which reflect their state of growth activity and degree of differentiation.

¹ This investigation was supported in part by Grants CA-10893, P2, and CA-21520 from the National Cancer Institute of the Department of Health and Human Services and by Grant PDT-109 from the American Cancer Society.

² To whom requests for reprints should be addressed.

Received 9/19/83. Accepted 12/ 6/83.

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