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DNA Damage Induced in Human Diploid Cells by Decay of Incorporated Radionuclides¹

Laboratory of Radiobiology, Harvard School of Public Health, Boston, Massachusetts 02115

Alkaline and neutral elution techniques were used to characterize the production of single- and double-strand DNA breaks in human diploid fibroblasts by incorporated radionuclides. ¹²⁵I was incorporated in DNA as [¹²⁵I]iododeoxyuridine, ³H as [³H]-thymidine, and ¹⁴C as [¹⁴C]thymidine. Under frozen conditions, ¹²⁵I was 3 times as efficient as ³H per decay in inducing single-strand breaks and 6 times as efficient as ¹⁴C. For double-strand break production, however, ¹²⁵I was 6 times as efficient per decay as ³H. It was calculated that, on the average, each ¹²⁵I decay produces about one double-strand break in the frozen state. Under nonfrozen conditions, ¹²⁵I and external X-rays were roughly 5-fold and ³H about 3-fold more efficient in double-strand break induction than under frozen conditions.

¹ This work was supported by Contract EVO4322.A004 from the United States Department of Energy and by Training Grant CA-09078 and Center Grant ES-00002 from the NIH.

² Present address: Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02139.

³ To whom requests for reprints should be addressed.

Received 3/29/83. Accepted 12/15/83.

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