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Stimulation of Glycolysis by Placental Polypeptides and Inhibition by Duramycin¹

Section of Biochemistry, Molecular and Cell Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853

Placental polypeptides present in crude preparations of transforming growth factors stimulate glycolysis when added to quiescent 3T3 cells, normal rat kidney, and chick embryo fibroblasts. The stimulation was apparent over a time period of at least 90 min and was seen at glucose concentrations ranging from 1 to 30 mM. Duramycin, an antibiotic isolated from Streptomyces cinnamomeus, inhibits the polypeptide-stimulated and nonstimulated glycolysis of intact cells, since it permeabilizes cells to P_i and nucleotides. However, duramycin also inhibits the Na⁺-K⁺-ATPase as well as the ouabain-insensitive Mg²⁺-ATPase of plasma membranes. Duramycin has no effect on glycolysis catalyzed by cell-free extracts of Ehrlich ascites tumor cells in the presence of mitochondrial ATPase but partially inhibits glycolysis when ADP and P_i are generated by ATPases of plasma membrane preparations.

¹ This investigation was supported by Grant BC-156 from the American Cancer Society.

² To whom requests for reprints should be addressed, at Section of Biochemistry, Wing Hall, Cornell University, Ithaca, NY 14853.

Received 7/11/83. Accepted 12/20/83.