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Differential Effect of Hyperthermia on Murine Bone Marrow Normal Colony-forming Units and AKR and L1210 Leukemia Stem Cells¹

Division of Medical Oncology, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201

Thermal dose-survival curves for normal hematopoietic and leukemia cells were assessed by spleen colony assays after in vitro heat exposure ranging from 41° to 45°. No effect of 43° heat treatment on the fraction of cells lodging in the spleen was observed. Marked differences in heat sensitivity were observed between normal, L1210, and AKR leukemia cells, the first being less sensitive than were the malignant cells. Furthermore, a greater relative difference between normal stem cells and leukemia cells was observed at lower temperatures. Normal bone marrow cells forced into regenerative activity prior to heat treatment were more heat sensitive than was their undisturbed counterpart, suggesting that noncycling hematopoietic cells are less heat sensitive than are proliferating cells.

¹ Supported in part by CA 34144 and CA 34535 from the National Cancer Institute, NIH, Department of Health and Human Services.

² Supported in part by the Studienstiftung des Deutschen Volkes.

³ Supported by the Deutsche Krebshilfe e.V.

⁴ To whom requests for reprints should be addressed.

Received 8/13/82. Accepted 1/26/84.