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Transformation of Human Cells by DNA Transfection¹

Biology Department, Brookhaven National Laboratory, Upton, New York 11973

Normal human cells can be transformed to anchorage-independent growth by transfection with DNA from MOLT-4 lymphoblasts, derived from a patient with acute lymphocytic leukemia. Cells were treated with polyethylene glycol 6000, and DNA was administered as a calcium phosphate coprecipitate. The transforming activity of MOLT DNA was inactivated by treatment with DNase or by the restriction enzymes BglI or BamHI but not by EcoRV. DNA from transfected colonies can transform human recipient cells to anchorage-independent growth; the transforming ability and anchorage independence are maintained stably for at least 30 generations.

¹ This research was supported by a grant from the National Cancer Institute, Department of Health and Human Services (CA 26492) and by the Department of Energy.

² To whom requests for reprints should be addressed.

Received 11/17/83. Accepted 3/27/84.

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