This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hubbell, H. R. Articles by Maxwell, B. L. Search for Related Content PubMed PubMed Citation Articles by Hubbell, H. R. Articles by Maxwell, B. L.

Independent Sensitivity of Human Tumor Cell Lines to Interferon and Double-Stranded RNA¹

The Barry Ashbee Leukemia Research Laboratories, Department of Hematology/Oncology, Hahnemann University, Philadelphia, Pennsylvania 19102 [H. R. H., R-S. L.], and Department of Clinical Immunology and Biological Therapy, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030 [B. L. M.]

The antiproliferative effect of human interferons (IFNs) and double-stranded RNAs (dsRNAs) was measured in eight human tumor cell lines, five of which were derived from carcinomas of the bladder. Dose-response curves were generated for a 72-hr treatment period. The concentration of interferon or dsRNA necessary to inhibit tumor cell growth 50% compared to untreated cells was generated by linear regression analysis of the dose-response data. In the seven of eight cell lines in which a direct comparison could be made, IFN-ß was a more potent inhibitor than IFN-. Polyriboinosinic acid·polyribocytidylic acid consistently gave an increased antiproliferative response compared to its mismatched analogue, rl_n·r(C₁₂,U)_n. Correlations could not be made between either IFN- or IFN-ß and the dsRNA effect. No correlation was seen between IFN or dsRNA sensitivity and cell type, ability to bind IFN, growth rate, or tumorigenicity in nude mice.

The antiproliferative effect of dsRNA was studied in the presence of antibodies against IFN-ß in HT1080 Cl 4, a cell line sensitive to both IFN and dsRNA, and A2182, a cell line relatively resistant to IFN-ß but sensitive to dsRNA. In both cell lines, the anti-IFN-ß antibodies inhibited the antiproliferative effect of the dsRNAs. After treatment with a concentration of dsRNA necessary to inhibit tumor cell growth 50% compared to untreated cells, a concentration of IFN-ß necessary to inhibit tumor cell growth 50% was induced in the HT1080 Cl 4 cells; however, only a low level of IFN-ß was detected in the culture medium of the A2182 cells.

¹ This work was supported in part by USPHS Grant P01 CA-29545 from the National Cancer Institute and by the Interferon Research Fund through M. D. Anderson Hospital and Tumor Institute.

² To whom requests for reprints should be addressed, at The Barry Ashbee Leukemia Research Laboratories, Department of Hematology/Oncology, Hahnemann University, 230 North Broad Street, Philadelphia, PA 19102.

³ Present address: Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.

Received 4/27/83. Accepted 4/13/84.

This article has been cited by other articles:

				D. Gomez and N. C. Reich Stimulation of Primary Human Endothelial Cell Proliferation by IFN J. Immunol., June 1, 2003; 170(11): 5373 - 5381. [Abstract] [Full Text] [PDF]

				M. S. Chacko and M. L. Adamo Double-Stranded Ribonucleic Acid Decreases C6 Rat Glioma Cell Numbers: Effects on Insulin-Like Growth Factor I Gene Expression and Action Endocrinology, October 1, 2000; 141(10): 3546 - 3555. [Abstract] [Full Text] [PDF]

				C. Rozera, D. Carlei, P. L. Lollini, C. De Giovanni, P. Musiani, E. Di Carlo, F. Belardelli, and M. Ferrantini Interferon (IFN)-ß Gene Transfer into TS/A Adenocarcinoma Cells and Comparison with IFN-{alpha} : Differential Effects on Tumorigenicity and Host Response Am. J. Pathol., April 1, 1999; 154(4): 1211 - 1222. [Abstract] [Full Text] [PDF]