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Inhibitory Activity on Murine Granulocytic Colony Formation of Bone Marrow Cell-conditioned Medium Obtained from Colony-stimulating Factor-producing Tumor-bearing Nude Mice¹

Division of Hemopoiesis, Institute of Hematology [K. M., Y. I., K. H., S. S., M. S.], and Department of Internal Medicine [K. I., S. K., Y. M.], Jichi Medical School, Tochigi-Ken, 329-04, Japan

The effects of bone marrow-conditioned medium obtained from colony-stimulating factor-producing tumor-bearing nude mice (G-BM-CM) on mouse and human granulocyte-macrophage colony formation and mouse erythroid colony and burst formation were studied. Addition of G-BM-CM into the mouse granulocyte-macrophage colony-forming system containing colony-stimulating activity more strongly inhibited granulocyte colony formation than did mixed granulocyte-macrophage and macrophage colony formation, while it did not change the number of granulocyte colonies formed by human bone marrow cells stimulated by human granulocyte colony-stimulating activity. Addition of G-BM-CM slightly increased mouse erythroid colony and burst numbers when it was added into an erythroid colony-forming system stimulated by erythropoietin (1 unit/ml), and into the erythroid burst-forming system stimulated by erythropoietin (1 unit/ml) and 7% spleen cell-conditioned medium. These results might indicate that G-BM-CM mainly blocked commitment of mouse granulocyte-macrophage colony-forming cells to granulocytic progeny.

¹ Supported in part by Grants-in-aid for Cancer Research from the Ministry of Education, Science and Culture, Japan.

² To whom requests for reprints should be addressed, at Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minami-Kawachi-Machi, Kawachi-Gun, Tochigi-Ken, 329-04, Japan.

Received 1/ 6/84. Accepted 5/ 9/84.