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Elevated Growth Factor Levels in Transformed Mouse Embryo Cells Treated with N,N-Dimethylformamide¹

Bristol-Baylor Laboratory, Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030

The transformed mouse mebryo fibroblast cell line AKR-MCA, produces several transforming growth factor (TGF) activities which can be identified in cell extracts and serum-free conditioned medium. Treatment of these transformed cells with 1% N,N-dimethylformamide resulted in a more normal phenotype and an increased level of TGFs in cell extracts and conditioned medium. In addition, an 11-fold increase in an epidermal growth factor receptor-competing activity was observed in cell extracts and conditioned medium compared to control untreated cells. Fractionation of the conditioned medium on Bio-Gel P-100 showed that the same size classes of TGFs were present in N,N-dimethylformamide-treated as well as untreated cells. The increased EGF-receptor-competing activity was eluted in two peaks at M, 6500 and M, 4000. The M, 6500 peak did not coelute with a TGF peak in the N,N-dimethylformamide-treated cells.

¹ Supported by Grants PDT-109 from the American Cancer Society, CA-34432 from the NIH, and RR-05425 from USPHS.

² To whom requests for reprints should be addressed.

Received 12/ 5/83. Accepted 9/21/84.