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Detection of Benzo(a)pyrene:DNA Adducts in Human White Blood Cells

Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland 21201 [A. K. M. S.]; Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland 20205 [N. T. S., C. C. H.]; Institute of Occupational Health, Haartmaninkatu 1, Helsinki, Finland [K. H.]; and Mount Sinai School of Medicine, New York, New York 10024 [R. R. B.]

Metabolic activation of benzo(a)pyrene (BP) to its ultimate carcinogenic form, 7ß,8-diol-9,10-benzo(a)pyrene epoxide (BPDE), and the binding of BPDE to DNA are important steps in BP carcinogenicity in experimental animals. Since people of certain occupations are exposed to high concentrations of BP, we have used enzyme-linked immunosorbent assay and ultrasensitive enzymatic radioimmunoassay to measure BPDE:DNA adducts in white blood cells from 2 of these occupational groups. Seven of 28 samples from roofers and 7 of 20 samples from foundry workers were positive for BPDE:DNA adducts (range, 2 to 120 fmol BPDE/50 µg DNA). In a group of nine volunteers without these industrial exposures to BP, the two positive DNA samples were from cigarette smokers. Control DNA obtained from human lymphocyte cell line RPMI 4265 was negative. These results indicate that the metabolic activation of BP and formation of BPDE:DNA adducts occurs in humans.

¹ To whom requests for reprints should be addressed, at National Cancer Institute, Laboratory of Human Carcinogenesis, Building 37, Room 2C09, Bethesda, MD 20205.

Received 10/20/82. Accepted 9/21/84.

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