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Calcitonin Stimulation of Cyclic Adenosine 3':5'-Monophosphate Production with Growth Inhibition in Human Renal Adenocarcinoma Cell Lines

Third Division, Department of Medicine, Kobe University School of Medicine, 5-1 Kusunoki-cho, 7-Chome, Chuo-ku, Kobe 650, Japan

Responsiveness of cyclic adenosine 3':5'-monophosphate (cAMP) to parathyroid hormone, calcitonin, and vasopressin was studied in six human renal adenocarcinoma cell lines. Four of six renal adenocarcinoma cell lines showed increased cAMP content in response to calcitonin while the other two did not. Neither parathyroid hormone nor vasopressin increased the concentration of cAMP in each of these cell lines. The growth rate of KU-2 cells, which responded to calcitonin with an increase of cAMP content, was inhibited by calcitonin. On the other hand the growth rate of calcitonin-nonsensitive KH-39 cells was unaltered. The growth inhibitory effect of the hormone on KU-2 cells could be considered to be mediated by the increased cAMP levels from the following results: (a) there was positive correlation between the cellular cAMP content and growth inhibition after various amounts of calcitonin addition; (b) KU-2 growth was also suppressed by N⁶,O^2'-dibutyryl cAMP; and (c) a group of KU-2 cells which had become resistant to calcitonin-induced growth inhibition showed a diminished cAMP increase in response to calcitonin.

¹ To whom requests for reprints should be addressed.

Received 11/16/84. Revised 6/17/85. Accepted 6/25/85.