| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 [M. H., J. T., J. L. B., D. H., H. K.], and the Pigmented Lesion Study Group [D. E. E., E. B., D. G., W. H. C.] and the Departments of Human Genetics [G. B.] and Pathology and Laboratory Medicine [P. N.], University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Normal melanocytes and melanocytes of normal nevi, primary melanoma in the radial (RGP) and vertical (VGP) growth phases, and metastatic melanoma exhibited and maintained phenotypic differences when grown in tissue culture or in experimental animals. Only metastatic and VGP primary melanoma cells were tumorigenic in athymic nude mice and had nonrandom chromosomal abnormalities involving chromosomes 1, 6, and 7. The colony-forming efficiency in soft agar was also highest in these two cell types. A cell line of RGP primary melanoma had characteristics of both benign and malignant cells: nevus-like morphology; nontumorigenicity in nude mice; but karyotypic abnormality of chromosome 6. It also had a ganglioside pattern similar to that of normal melanocytes but not melanomas, i.e., a high GM3 ganglioside content compared to the amounts of GM2, GD2, and GD3 gangliosides. Binding of monoclonal antibodies secreted by hybridomas generated by immunization of mice with VGP primary and metastatic melanoma was highest with cells and supernatants of cultures from advanced melanoma and least with nevus cells. There was no binding to normal melanocytes except with the monoclonal antibodies specific for nerve growth factor receptor or 9-O-acetyl-GD3 ganglioside. On the other hand, monoclonal anti-nevus antibodies bound to melanocytes, nevus cells, and RGP primary melanoma cells but not to VGP primary or metastatic melanoma cells. Cultured human melanocytic cells appear to be a unique model for the study of tumor progression.
1 This work was supported by NIH Grants CA-25874, CA-21124, CA-10815, CA-29200, and CA-25298.
2 To whom requests for reprints should be addressed.
Received 4/29/85. Revised 8/ 8/85. Accepted 8/12/85.
This article has been cited by other articles:
![]() |
K. Araki, T. Shimura, T. Yajima, S. Tsutsumi, H. Suzuki, K. Okada, T. Kobayashi, A. Raz, and H. Kuwano Phosphoglucose Isomerase/Autocrine Motility Factor Promotes Melanoma Cell Migration through ERK Activation Dependent on Autocrine Production of Interleukin-8 J. Biol. Chem., November 20, 2009; 284(47): 32305 - 32311. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Lindegaard, P. Isager, J. U. Prause, and S. Heegaard Optic nerve invasion of uveal melanoma: clinical characteristics and metastatic pattern. Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3268 - 3275. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. A. Charles, V. S. K. Yee, S. W. Dusza, A. A. Marghoob, S. A. Oliveria, A. Kopf, D. Rigel, and A. C. Halpern Variation in the Diagnosis, Treatment, and Management of Melanoma In Situ: A Survey of US Dermatologists Arch Dermatol, June 1, 2005; 141(6): 723 - 729. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Denkins, J. Reiland, M. Roy, N. D. Sinnappah-Kang, J. Galjour, B. P. Murry, J. Blust, R. Aucoin, and D. Marchetti Brain metastases in melanoma: Roles of neurotrophins Neuro-oncol, April 1, 2004; 6(2): 154 - 165. [Abstract] [PDF] |
||||
![]() |
K. Bernard, E. Litman, J. L. Fitzpatrick, Y. G. Shellman, G. Argast, K. Polvinen, A. D. Everett, K. Fukasawa, D. A. Norris, N. G. Ahn, et al. Functional Proteomic Analysis of Melanoma Progression Cancer Res., October 15, 2003; 63(20): 6716 - 6725. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Satyamoorthy, G. Li, M. R. Gerrero, M. S. Brose, P. Volpe, B. L. Weber, P. van Belle, D. E. Elder, and M. Herlyn Constitutive Mitogen-activated Protein Kinase Activation in Melanoma Is Mediated by Both BRAF Mutations and Autocrine Growth Factor Stimulation Cancer Res., February 15, 2003; 63(4): 756 - 759. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. F. Goldberg, M. E. Miele, N. Hatta, M. Takata, C. Paquette-Straub, L. P. Freedman, and D. R. Welch Melanoma Metastasis Suppression by Chromosome 6: Evidence for a Pathway Regulated by CRSP3 and TXNIP Cancer Res., January 15, 2003; 63(2): 432 - 440. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Satyamoorthy, G. Li, B. Vaidya, J. Kalabis, and M. Herlyn Insulin-like Growth Factor-I-induced Migration of Melanoma Cells Is Mediated by Interleukin-8 Induction Cell Growth Differ., February 1, 2002; 13(2): 87 - 93. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Berking, R. Takemoto, H. Schaider, L. Showe, K. Satyamoorthy, P. Robbins, and M. Herlyn Transforming Growth Factor-{beta}1 Increases Survival of Human Melanoma through Stroma Remodeling Cancer Res., November 1, 2001; 61(22): 8306 - 8316. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Satyamoorthy, G. Li, B. Vaidya, D. Patel, and M. Herlyn Insulin-like Growth Factor-1 Induces Survival and Growth of Biologically Early Melanoma Cells through Both the Mitogen-activated Protein Kinase and {beta}-Catenin Pathways Cancer Res., October 1, 2001; 61(19): 7318 - 7324. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Satyamoorthy, N. H. Chehab, M. J. F. Waterman, M. C. Lien, W. S. El-Deiry, M. Herlyn, and T. D. Halazonetis Aberrant Regulation and Function of Wild-Type p53 in Radioresistant Melanoma Cells Cell Growth Differ., September 1, 2000; 11(9): 467 - 474. [Abstract] [Full Text] |
||||
![]() |
F. Meier, M. Nesbit, M.-Y. Hsu, B. Martin, P. Van Belle, D. E. Elder, G. Schaumburg-Lever, C. Garbe, T. M. Walz, P. Donatien, et al. Human Melanoma Progression in Skin Reconstructs : Biological Significance of bFGF Am. J. Pathol., January 1, 2000; 156(1): 193 - 200. [Abstract] [Full Text] [PDF] |
||||
![]() |
M.-Y. Hsu, D.-T. Shih, F. E. Meier, P. Van Belle, J.-Y. Hsu, D. E. Elder, C. A. Buck, and M. Herlyn Adenoviral Gene Transfer of ß3 Integrin Subunit Induces Conversion from Radial to Vertical Growth Phase in Primary Human Melanoma Am. J. Pathol., November 1, 1998; 153(5): 1435 - 1442. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. H. Kraemer, M. Herlyn, S. H. Yuspa, W. H. Clark Jr, G. K. Townsend, G. R. Neises, and V. J. Hearing Reduced DNA Repair in Cultured Melanocytes and Nevus Cells From a Patient With Xeroderma Pigmentosum Arch Dermatol, February 1, 1989; 125(2): 263 - 268. [Abstract] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |