This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by King, R. J. B. Articles by Taylor, R. W. Search for Related Content PubMed PubMed Citation Articles by King, R. J. B. Articles by Taylor, R. W.

Histochemical Studies with a Monoclonal Antibody Raised against a Partially Purified Soluble Estradiol Receptor Preparation from Human Myometrium

Hormone Biochemistry Department [R. J. B. K., A. I. C., K. L.] and Pathology Service [J. G.], Imperial Cancer Research Fund, P. O. Box 123, Lincoln's Inn Fields, London WC2A 3PX; Imperial Cancer Research Fund, Breast Cancer Unit, Guy's Hospital, London SE1 9RT [R. N., R. M.]; and Department of Gynaecology, St. Thomas's Hospital Medical School, London SE1 7EH [S. R., R. W. T.], United Kingdom

A monoclonal antibody (D5) raised against affinity-purified cytosol estradiol receptor (REC) from human myometrium has been used to stain human tissues by means of an indirect immunoperoxidase method. Good staining was obtained with ethanol-, glutaraldehyde-, or Carnoy's-fixed material but not with formalin or Bouin's fixation. Cytoplasmic staining of human breast tumors exhibited a highly significant correlation (P < 0.001) with REC assayed by conventional estradiol-binding assay provided that allowance was made for both staining intensity and cellularity of the tumor; no correlation existed with soluble progesterone receptor content. Both patient age and tumor differentiation influenced staining patterns in the same way as did REC content. Cultured REC-positive human breast tumor cell lines (MCF-7, ZR-75-1, and CA-2) showed positive staining as did cultured epithelium from human milk. Epithelia in normal breast and fibroadenoma exhibited variable staining that rarely reached the intensity seen in REC-positive tumor cells.

The staining patterns of human normal endometrium, myometrium, fallopian tube, ectocervix, endocervix, and ovary and neoplastic endometrium and ovary are described. In every situation thus far examined only cytoplasmic staining has been observed.

¹ To whom requests for reprints should be addressed.

Received 2/13/85. Revised 6/28/85. Accepted 7/17/85.

This article has been cited by other articles:

				K. Ishihama, M. Yamakawa, S. Semba, H. Takeda, S. Kawata, S. Kimura, and W. Kimura Expression of HDAC1 and CBP/p300 in human colorectal carcinomas J. Clin. Pathol., November 1, 2007; 60(11): 1205 - 1210. [Abstract] [Full Text] [PDF]