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Histopathological Comparison of the Effects of Hematoporphyrin Derivative on Two Different Murine Tumors Using Computer-enhanced Digital Video Fluorescence Microscopy¹

Departments of Developmental and Cell Biology and Surgery and Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, California 92717

A comparison study of the effects of hematoporphyrin derivative (HPD) photoradiation therapy on two different mouse sarcoma tumor model systems (RIF-1 + EMT-6) was performed. Twenty-four h after i.v. administration of HPD, the responses to total laser light doses of 50–400 J/cm² were evaluated by histological examination and the uptake and distribution of HPD using a computer enhanced digital video fluorescence microscopy technique.

In response to total laser light dose (630 nm) of 50–400 J/cm², 40 mice with RIF-1 tumor showed only minimal superficial tumor necrosis upon histological examination and a 9–12% increase in maximal tissue fluorescence. In contrast, 40 mice with EMT-6 tumor showed marked areas of patchy coagulation necrosis and vascular hemorrhage at doses as low as 50 J/cm² and essentially total tumor destruction at total light doses of 150 J/cm² or more. A 59–74% increase in maximal tissue fluorescence was observed using digital video fluorescence microscopy.

It is concluded that the greater efficacy of treatment in the EMT-6 tumor as compared to the RIF-1 tumor was due to the greater localization of HPD as demonstrated by digital video fluorescence microscopy.

¹ This research was supported by NIH Grants RRO 1192 and CA 32248.

² To whom requests for reprints should addressed, at Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92717.

Received 12/27/84. Revised 7/24/85. Accepted 7/30/85.