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Division of Cell Biology, The Netherlands Cancer Institute (Antoni van Leeuwenhoekhuis), 121 Plesmanlaan, 1066CX Amsterdam, The Netherlands [E. R., G. La R., J. G. C., M. J. S.], and Institute for Molecular Biology, Vrije Universiteit Brussel, Paardenstraat 65, 1640 Sint Genesius-Rode, Belgium [P. De B.]
BW5147 lymphoma cells, which are noninvasive and nonmetastatic, were fused with normal T-lymphocytes. The invasiveness of the generated T-cell hybridomas was tested in hepatocyte cultures, and their metastatic potential was tested by tail vein injection. A total of 29 hybridomas generated from alloantigen-activated T-cells were all found to be invasive. One of these cell lines rapidly lost invasiveness in culture. Most hybridomas generated from nonstimulated spleen T-cells were also invasive, but 5 of 27 were not. Six invasive and four noninvasive hybridomas were injected into the tail vein of syngeneic mice. All invasive cell lines caused extensive and widespread tumor growth, particularly in the liver, which was usually severalfold enlarged; the spleen; kidneys; and ovaries. In contrast the noninvasive hybrids, which were tumorigenic upon s.c. injection, did not form any metastases. We conclude that properties derived from normal T-cells, when introduced into noninvasive T-lymphoma cells, cause them to become invasive as well as metastatic. Furthermore for this tumor cell type invasiveness as measured in hepatocyte cultures appears to be closely associated with the ability to colonize organs from the bloodstream.
1 To whom requests for reprints should be addressed.
2 Supported by Grant NKI 83-13 from the KWF/Netherlands Cancer Foundation.
Received 1/10/85. Revised 7/ 2/85. Accepted 7/26/85.
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