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Flow Cytometric Analysis of Pentakis(aziridino)thiatriazadiphosphorine Oxide (SOAz)-induced Changes in Cell Cycle Progression of HeLa and HL-60 Cells

Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, 15 rue de l'Ecole de Médecine [C. H., X. R., M. A.] and Laboratoire d'Hématologie, 4 avenue de l'Observatoire [J. N.], 75006 Paris, and Service d'Hématologie, Hotel-Dieu, 1 Place du Parvis Notre-Dame, 75004 Paris [J-P. M.], France

The treatment of HeLa and HL-60 cells with various concentrations of pentakis(arizidino)thiatriazadiphosphorine oxide results in inhibition of growth and modification of cell cycle distribution. These phenomena were observed at 10^-4 M and 5 x 10^-5 M for HeLa cells and 10^-5 M and 5 x 10^-6 M for HL-60 cells. The estimation of DNA content by flow cytometry showed an important shift in the distribution of cycling cells with a striking arrest in G₂ for both cell lines with a concomitant late S-phase accumulation for HeLa cells. Incubation of cells in drug-free medium 3 days after treatment did not show any change in DNA distribution, suggesting the irreversibility of drug action.

¹ To whom requests for reprints should be addressed.

Received 6/20/84. Accepted 10/25/84.