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Potentiation of Cytotoxic Activity of Immunotoxins on Cultured Human Cells

Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis, National Cancer Institute, NIH, Bethesda, Maryland 20205

The cytotoxic activity against human tumor cells of toxic conjugates of Pseudomonas exotoxin with anti-transferrin receptor antibody or epidermal growth factor was potentiated up to 10 to 20-fold by the calcium antagonists verapamil, D-600, and diltiazem and by the lysosomotropic agent ß-glycylphenylnaphthylamide. The potentiating activity of these agents could be predicted by measuring the inhibition of protein synthesis by the immunotoxins on various cell lines. The use of potentiating agents such as these in combination with immunotoxins may prove useful in the treatment of some human cancers.

¹ To whom requests for reprints should be addressed, at Molecular Cell Genetics Section, Laboratory of Molecular Biology, National Cancer Institute, NIH, Building 37, Room 2E22, Bethesda, MD 20205.

Received 8/23/84. Accepted 12/ 4/84.