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Oxygen Dependence of Hematoporphyrin Derivative-induced Photoinactivation of Chinese Hamster Cells

Radiation Oncology Branch, Division of Cancer Treatment, National Cancer Institute, NIH, Bethesda, Maryland 20205

The oxygen dependence of hematoporphyrin derivative (HPD)-induced photoinactivation of Chinese hamster V79 cells was examined. Cells were treated with HPD (25 µg/ml) for 2 h and subsequently exposed to red light (>590 nm) under either aerated or hypoxic (<10 ppm O₂) conditions. Hypoxic cells were found to be extremely resistant to the lethal effects of HPD and light. The electron-affinic X-ray hypoxic cell sensitizer, SR-2508, did not sensitize hypoxic HPD-treated cells to light. The clinical implications of these findings are discussed, with consideration of the possibility that hypoxic areas in tumors may limit HPD phototherapy.

¹ To whom requests for reprints should be addressed, at Radiation Oncology Branch, National Cancer Institute, Building 10, Room B3B69, 9000 Rockville Pike, Bethesda, MD 20205.

Received 10/17/84. Revised 1/ 7/85. Accepted 2/ 4/85.

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