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Measurements of in Vivo ³¹P Nuclear Magnetic Resonance Spectra in Neuroectodermal Tumors for the Evaluation of the Effects of Chemotherapy¹

Department of Neurosurgery [S. N., Y. H., C. T., T. H., S. U., K. H.], and Physiology [H. N.], Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-Ku, Kyoto 602, and Department of Molecular Physiology, National Institute for Physiological Science, Aichi 444 [H. W.], Japan

The effects of chemotherapy on living tumor tissue in hamsters and rats were investigated by measuring the ³¹P nuclear magnetic resonance spectra using topical magnetic resonance. Human neuroblastoma, human glioblastoma, and rat glioma tumor cells were inoculated s.c. in the lumbar region of the animals. After the diameter of the tumors increased to 1.5 cm, in vivo ³¹P nuclear magnetic resonance spectra were measured selectively in the tumors with a TMR-32 spectrometer. Adenosine triphosphate, inorganic phosphate (P_i), phosphodiester, and phosphomonoester peaks were observed. The phosphocreatine peak was hardly detectable, adenosine triphosphate and phosphomonoester peaks were high, and tissue pH, calculated from the chemical shift of P_i, declined. Regardless of the tumor origin or the histological type, the spectral pattern of each neuroectodermal tumor was found to be essentially the same. After i.v. injection of a large dose of a chemotherapeutic agent, adenosine triphosphate peaks decreased and P_i increased gradually, resulting in a dominant P_i peak pattern after 6 to 12 hours. However, during the same period, there were no observable changes in the spectra of normal organs. These findings indicated that the drugs have a selective and direct action on the energy metabolism of tumor cells. With lower drug doses, no remarkable changes were seen in the spectrum. Measurement of in vivo ³¹P nuclear magnetic resonance spectra is valuable not only to investigate the energy metabolism in tumor tissue but also to evaluate the effects of chemotherapy.

¹ Supported in part by Grants-in-Aid 58440059, 58570597, and 59570623 for Scientific Research from the Ministry of Education, Science and Culture of Japan.

² To whom requests for reprints should be addressed.

Received 5/22/84. Revised 10/22/84. Revised 1/23/85. Accepted 2/13/85.