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Transferrin-like Activity Produced by Murine Malignant T-Lymphoma Cell Lines¹

Laboratory of Biomedical and Environmental Sciences [E. F. H.] and Department of Medicine [S. K., E. F. H.], University of California, Los Angeles, California 90024

Transferrin has been considered to be an essential requirement for hematopoietic cell proliferation in culture. We have isolated two cloned lymphoma cell lines, SL 12.1 and SL 12.4, which grow and adapt in serum-free medium without added transferrin. Antibody to the transferrin receptor blocks the growth of these cells. We have also demonstrated that transferrin-free conditioned medium from the cells will compete with transferrin for binding. Furthermore, conditioned medium from SL 12.1 and SL 12.4 cells induces and supports exponential growth of a transferrin-dependent lymphoma cell line, SL 12. We conclude that these two transferrin-independent cloned lines produce transferrin-like activity which plays a crucial role for cell proliferation.

¹ Operated for the United States Department of Energy by the University of California under Contract DE-AC03-76-SF00012. This manuscript was supported by the Director of the Office of Energy Research, Office of Health and Environmental Research. This investigation was also supported by USPHS Grant CA 12386, awarded by the National Cancer Institute, Department of Health and Human Services, and Cancer Center Support Grant CA 16042.

² To whom requests for reprints should be addressed, at UCLA Warren Hall, 900 Veteran Avenue, Los Angeles, CA 90024.

Received 10/22/84. Revised 5/ 9/85. Accepted 5/10/85.