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Effects of Butylated Hydroxyanisole on the Tumorigenicity and Metabolism of N-Nitrosodimethylamine and N-Nitrosopyrrolidine in A/J Mice¹

Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, New York 10595

Female A/J mice were maintained on NIH-07 diet or on NIH-07 diet containing butylated hydroxyanisole (BHA, a mixture of 2- and 3-tert-butyl-4-hydroxyanisole), 5 mg/g, for 1 week prior to and during 10 weeks of treatment with N-nitrosodimethylamine (NDMA) or N-nitrosopyrrolidine (NPYR), administered in the drinking water. Twenty weeks after nitrosamine treatment ended, mice were sacrificed and lung adenomas were counted. BHA inhibited NDMA tumorigenesis but enhanced NPYR tumorigenesis. Treatment of A/J mice for three weeks with BHA (5 mg/g) added to semisynthetic diet increased whole lung microsomal -hydroxylation of NDMA and NPYR, as measured by aldehyde production, and increased lung and hepatic glutathione-S-transferase activities. No evidence was found for formation of S-methylglutathione in incubations with NDMA and hepatic supernatants obtained from BHA treated or control mice. Four h after gavage of NDMA, levels of 7-methylguanine in the lung DNA of mice treated with BHA were higher than in the lung DNA of control mice, but levels of O⁶-methylguanine in the two groups were the same. The results of this study indicate that BHA treatment increases the microsomal metabolic hydroxylation of both NDMA and NPYR, but has differential effects on their tumorigenic activities.

¹ Supported by National Cancer Institute Grant CA-32519. This is Paper 2 in "Dietary Inhibitors of Chemical Carcinogenesis."

² To whom requests for reprints should be addressed.

Received 5/22/85. Revised 9/20/85. Accepted 10/ 8/85.