This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mizoguchi, A. Articles by Sugiyama, T. Search for Related Content PubMed PubMed Citation Articles by Mizoguchi, A. Articles by Sugiyama, T.

Cell Surface Asparagine-linked Sugar Chains of Human Early Myeloblastic Leukemic Cells (KG-1a)¹

Departments of Anatomy [A. M.] and Pathology [S. M., N. S., H. Y., T. S.], Kobe University School of Medicine, Chuo-ku, Kobe, Japan

The asparagine-linked sugar chains obtained from total cell surface membrane glycoproteins of human early myeloblastic leukemic cells (KG-1a cells) were studied. The sugar chains liberated by hydrazinolysis were purified by paper electrophoresis, paper chromatography, and Bio-Gel P-4 chromatography followed by analysis of exoglycosidase digestion and methylation study. Neutral oligosaccharides were all composed of high mannose type sugar chains. Acidic oligosaccharides were chiefly composed of typical bi-, tri-, and tetraantennary complex type sugar chains with Galß14GlcNAcß1groups and NeuAc23 or 6Galß14GlcNAcß1 groups (in which Gal is galactosyl, GlcNac is N-acetylglucosamine, and NeuAc is N-acetylneuraminic acid) as side chains. Moreover the following two structures were identified (in which Fuc is fucosyl): monosialyl bi- and triantennary sugar chains with a Galß14(Fuc13)GlcNAcß1 group (X determinant) as one of the side chains; and monosialyl tetraantennary sugar chains with a Galß14GlcNAcß13Galß14GlcNAc group (repeating N-acetyllactosamine unit) as one of the side chains. These data together with our previous studies on sugar chains of K562 cells [early erythroblast], adult erythrocytes [H. Yoshima, N. Shiraishi, A. Matsumoto, S. Maeda, T. Sugiyama, and A. Kobata, J. Biochem. (Tokyo), 91: 233–246, 1982], and HL-60 cells [promyelocyte] [A. Mizoguchi, S. Takasaki, S. Maeda, and A. Kobata, J. Biol. Chem., 259: 11943–11957, 1984] strongly suggest that the cell surface asparagine-linked sugar chains alter in an orderly fashion, systematically in association with lineage and maturation stages during hematopoietic cell differentiation.

¹ This work was supported partly by the lue Memorial Foundation for Scientific Research; partly by grant from the Ministry of Education, Science and Culture of Japan (No 59570517); and partly by Hyogo Prefecture Anti-Cancer Association.

² To whom requests for reprints should be addressed.

Received 4/ 2/85. Revised 9/20/85. Accepted 9/23/85.