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1,25-Dihydroxyvitamin D₃-regulated Expression of Genes Involved in Human T-Lymphocyte Proliferation and Differentiation¹

Third Division, Departments of Medicine [T. M., Y. N., T. K., Y. K., T. F.] and Pathology [R. T., T. S.], Kobe University School of Medicine, Kobe 650, and Institute for Virus Research, Kyoto University [K. M.], Kyoto 606, Japan

1,25-Dihydroxyvitamin D₃ [1,25-(OH)₂D₃] inhibited the secretion of -interferon from human T-lymphocytes activated by the calcium ionophore, A23187, or phytohemagglutinin with or without 12-O-tetradecanoylphorbol-13-acetate. The agent also inhibited cell proliferation and interleukin 2 secretion by these cells. The inhibition of -interferon secretion was time and dose dependent and partially abolished by the addition of exogenous human recombinant interleukin 2. To elucidate the molecular events by which 1,25-(OH)₂D₃ inhibits cell proliferation and lymphokine secretion, complementary DNA probes were used to follow the expression of genes involved in human T-lymphocyte proliferation and differentiation. 1,25-(OH)₂D₃ inhibited the expression of interleukin 2 and -interferon messenger RNA in human lymphocytes activated by phytohemagglutinin and 12-O-tetradecanoylphorbol-13-acetate. It also inhibited the accumulation of c-myc protooncogene messenger RNA and, to a lesser extent, interleukin 2 receptor messenger RNA in these cells. However, it did not affect the expression of the HLA-DR gene. These results suggest that 1,25-(OH)₂D₃ selectively regulates T-lymphocyte activation-related genes at the level of messenger RNA.

¹ This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture and by a grant for cancer research from the Ministry of Health and Welfare of Japan.

² To whom requests for reprints should be addressed.

Received 12/19/85. Revised 5/ 1/86. Accepted 7/17/86.

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